Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: study not GLP but in which all parameters described are comparable to guideline methods for testing acute toxicity to fish
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Reference
Reference Type:
publication
Title:
Toxicity of Vanadium to Different Freshwater Organisms.
Author:
Beusen J.M. and Neven B.
Year:
1987
Bibliographic source:
Bulletin of Environmental Contamination and Toxicology, 39, 194-201.

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Deviations:
no
Remarks:
even if not all data are indicated
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
sodium metavanadate NaVO3
IUPAC Name:
sodium metavanadate NaVO3
Test material form:
not specified
Details on test material:
purity > 98%

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
not specified
Details on test solutions:
Dilution water used for stock cultures and tests is synthetic hard water:
composition (mg/L deionized water):
100 mg NaHCO3,
20 mg KHCO3,
180 mg MgSO4.7H2O
200 mg CaCl2.2H2O

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Brachydanio rerio are obtained from a commercial supply house.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
no data
Test temperature:
23°C +/-2°C
pH:
7.7 to 8.5
Dissolved oxygen:
above 6 mg/L
Salinity:
not given
Nominal and measured concentrations:
Not given, but the measured vanadium concentrations were within 10% of the initial amount of the metal added to each test vessel.
A control plus at least 5 concentrations were selected on a basis of a logarithmic expansion.
Details on test conditions:
Before testing, fishes from a single stock of similar lenght and age are adapted to laboratory conditions in 200 L tanks filled with hard water for at least 12 days. They were fed twice daily, ceasing 24h before initiation of the test.
photoperiod: 14h daylight/ 10h darkness

TEST SYSTEM
- Test vessel: 1L beakers filled with 1L of test solution
- fishes are transferred to new test medium after 48 and 96h
- Aeration: gentle bubble aeration
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): duplicate

Before testing, fishes from a single stock of similar lenght and age are adapted to laboratory conditions in 200 L tanks filled with hard water for at least 12 days. They were fed twice daily, ceasing 24h before initaition of the test.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 14h daylight/ 10h darkness

EFFECT PARAMETERS MEASURED: mortalities, at 24h intervals
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
4 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
element
Basis for effect:
mortality (fish)
Remarks on result:
other: geometric mean of 3 data
Details on results:
the 96h LC50 data are as follows:
2.9 (CI: 2.1- 3.4)
4.1 (CI: 2.6- 6.4)
5.3 (CI: 3.4- 8.4)

Applicant's summary and conclusion

Executive summary:

Zebrafish; Danio rerio were exposed to a control and at least 5 measured vanadium concentrations during 96h under static conditions and in a way similar to the guidelines. The 96h-LC50 for mortality was determined to be 4 mg V/L (geometric mean of 3 replicates).