Registration Dossier
Registration Dossier
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EC number: 269-535-7 | CAS number: 68296-59-3
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11 - 25 Jan 1977
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 977
- Report date:
- 1977
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Principles of method if other than guideline:
- Internal Guideline Hoechst AG
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Ethoxybisbenzimidazo[2,1-b:1',2'-j]benzo[lmn][3,8]phenanthroline-6,9-dione
- EC Number:
- 277-315-7
- EC Name:
- Ethoxybisbenzimidazo[2,1-b:1',2'-j]benzo[lmn][3,8]phenanthroline-6,9-dione
- Cas Number:
- 73195-14-9
- Molecular formula:
- C28H16N4O3
- IUPAC Name:
- 1-ethoxybisbenzimidazo[2,1-b:1',2'-j]benzo[lmn][3,8]phenanthroline-6,9-dione
- Test material form:
- liquid
- Details on test material:
- Vat Brown 57
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Hoechst AG - own breeding
- Age at study initiation: ca. 6 weeks
- Weight at study initiation: 88 to 96 g (females)
- Fasting period before study: 16 hours before to 2 hours after dosing
- Housing: in groups
- Diet: Altromin 1324 ad libitum
- Water: tap ad libitum
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 25% (250 mg/mL)
- Amount of vehicle (if gavage): 20 mL/kg - Doses:
- 5000 mg/kg bw
- No. of animals per sex per dose:
- 10 females
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: observation: clinical signs multiple times on day one, thereafter twice daily; body weight: weekly
- Necropsy of survivors performed: yes - Statistics:
- N/A
Results and discussion
- Preliminary study:
- NA
Effect levels
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 5 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- not determinable due to absence of adverse toxic effects
- Mortality:
- No mortality observed
- Clinical signs:
- other: No effects
- Gross pathology:
- No effects
- Other findings:
- Feces were brownish discolored in all animals.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The LD50 (oral, gavage) in female rats was determined to be >5000 mg/kg bw. The substance is not classifiable according to CLP criteria.
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