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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
of read across substance
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals
Reason / purpose:
read-across source
Reason / purpose:
read-across source
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
WoE report is based on two short term toxicity study of fish for the test chemical :
2.This study was designed to assess the toxic effects of the test compound on the Zebra fish (Danio rerio).
3.Bioassay was conducted to evaluate toxicity of test material on fish.
GLP compliance:
no
Analytical monitoring:
not specified
Vehicle:
not specified
Details on test solutions:
2. The test substance is insoluble in water as well as in the solvents namely, ethanol, acetone, acetonitrile, N,N-Dimethyl formamide, Tertiary butyl alcohol, chloroform. Therefore, the test substance was used as it is and test solution was prepared by dissolving 50 mg, 100 mg, & 150 mg of the test substance in 10 liters deionized water with continuous stirring for achieving test concentrations of 5 mg/L, 10 mg/L & 15 mg/L, respectively.
Test organisms (species):
other: 2. Danio rerio , 3.Oryzias latipes
Details on test organisms:
.TEST ORGANISM
- Common name: Zebra Fish
- Strain: Danio rerio
- Source: Ashish Fish Center, Dharampeth, Nagpur, MS (India)
- Age at study initiation (mean and range, SD): 3-4 months
- Length at study initiation (length definition, mean, range and SD): Average 3.63 cm
- Weight at study initiation (mean and range, SD): 0.399 g (approx.)
- Method of breeding: No data
- Feeding during test: Food was withdrawn 1 day before and during exposure
- Food type: Standard brand feed
- Frequency: Once daily

ACCLIMATION
- Acclimation period: seven days
- Acclimation conditions (same as test or not): same
- Type and amount of food: Standard brand feed
- Feeding frequency: Once in a day up to sixth day of acclimatization
- Health during acclimation (any mortality observed): During last 48 hours mortality was < 5 %

2.The fish were held in the laboratory at 20-23oC for 2-4 weeks prior to use in bioassay and were fed an excess of dry commercial diet pellet for trout daily. The Ssh were acclimated to the test system for 2 days prior to testing without feeding.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
2)25 milligrams per liter Calcium ca
Test temperature:
21- 36°C
pH:
6-8
Dissolved oxygen:
1) 7.4 mg/l
2)≥5-≥8.5 milligrams per liter
Nominal and measured concentrations:
1) 5 mg/L, 10 mg/L & 15 mg/L
Details on test conditions:
1) TEST SYSTEM
- Test vessel: The test vessels used were all glass bowl aquaria of 12 inch each.
- Type: open
- Material, size, headspace, fill volume:
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionized water was used

OTHER TEST CONDITIONS
Photoperiod: 12 hours light and 12-hour darkness

2)Ten fish were tested at each concentration which l'ncreases by geometrical progression of 1.4 in 3-5 / glass beaker held in constant temperature (21-22oC) water bath without feeding or artihcial aeration.
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
15 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Read across 1
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
2.4 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Read across 2
Validity criteria fulfilled:
not specified
Conclusions:
The test chemical is likely to be toxic to fish in the dose range of 2.4 - 15.0 mg/l . Since the additional criteria mentioned in the CLP regulation related to biodegradability and Log Kow is not satisfied thus the substance has not been considered for aquatic toxicity classification.
Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the short term toxicity of fish of the test chemical .The studiesare as mentioned below:

For structurally similar read across substance , a 96 h study was conducted for test material on Danio rerio, at various test concentrations of 5 mg/L, 10 mg/L & 15 mg/L, the fishes were found to be freely swimming in the bowl aquaria and no mortality were observed at test concentrations. No mortalities were found in the control aquaria. Based on nominal concentrations, experimental median lethal concentration [LC-50 (96 h)] for test material on Danio rerio was found to be >15 mg/L. But the substance was considered to be rapidly degradable and has no adverse effect on aquatic environment. Hence , it is concluded that test material does not exhibit short term toxicity to fish.

For , another structurally similar read across substance , the lethal concentration (LC50) value of test material in fish (Oryzias latipes) in a 48 hr study on mortality effect was found to be 2.4 mg/L. As the substance was found to be rapidly degradable. Since the additional criteria mentioned in the CLP regulation related to biodegradability is not satisfied thus the substance has not been considered for aquatic toxicity classification.

Eventhough the solubility of target in water is less , the target as well as the structurally similar read across substance are redialy biodegradable . Since the additional criteria mentioned in the CLP regulation related , biodegradability is not satisfied thus the substance has not been considered for aquatic toxicity classification.

Description of key information

Short term toxicity to fish:

Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the short term toxicity of fish of the test chemical .The studiesare as mentioned below:

For structurally similar read across substance , a 96 h study was conducted for test material on Danio rerio, at various test concentrations of 5 mg/L, 10 mg/L & 15 mg/L, the fishes were found to be freely swimming in the bowl aquaria and no mortality were observed at test concentrations. No mortalities were found in the control aquaria. Based on nominal concentrations, experimental median lethal concentration [LC-50 (96 h)] for test material on Danio rerio was found to be >15 mg/L.But the substance was considered to be rapidly degradable and has no adverse effect on aquatic environment. Hence , it is concluded that test material does not exhibit short term toxicity to fish.

For , another structurally similar read across substance , the lethal concentration (LC50) value of test material in fish (Oryzias latipes) in a 48 hr study on mortality effect was found to be 2.4 mg/L. As the substance was found to be rapidly degradable. Since the additional criteria mentioned in the CLP regulation related to biodegradability is not satisfied thus the substance has not been considered for aquatic toxicity classification.

Eventhough the solubility of target in water is less , the target as well as the structurally similar read across substance are redialy biodegradable . Since the additional criteria mentioned in the CLP regulation related , biodegradability is not satisfied thus the substance has not been considered for aquatic toxicity classification.

Key value for chemical safety assessment

LC50 for freshwater fish:
15 mg/L

Additional information

Short term toxicity to fish:

Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the short term toxicity of fish of the test chemical .The studiesare as mentioned below:

For structurally similar read across substance , a96 h study was conducted for test material on Danio rerio, at various test concentrations of 5 mg/L, 10 mg/L & 15 mg/L, the fishes were found to be freely swimming in the bowl aquaria and no mortality were observed at test concentrations. No mortalities were found in the control aquaria. Based on nominal concentrations, experimental median lethal concentration [LC-50 (96 h)] for test material on Danio rerio was found to be >15 mg/L.But the substance was considered to be rapidly degradable and has no adverse effect on aquatic environment. Hence , it is concluded that test material does not exhibit short term toxicity to fish.

For , another structurally similar read across substance , the lethal concentration (LC50) value of test material in fish (Oryzias latipes) in a 48 hr study on mortality effect was found to be 2.4 mg/L. As the substance was found to be rapidly degradable. Since the additional criteria mentioned in the CLP regulation related to biodegradability is not satisfied thus the substance has not been considered for aquatic toxicity classification.

Eventhough the solubility of target in water is less , the target as well as the structurally similar read across substance are redialy biodegradable . Since the additional criteria mentioned in the CLP regulation related , biodegradability is not satisfied thus the substance has not been considered for aquatic toxicity classification.