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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

The test item is not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

A ready biodegradation test, similar to the OECD 301B guideline, was conducted on N(B-hydroxyethyl)-2,4-diaminoanisole sulfate. The test material was tested at 40 mg active/L (15.5 mg TOC/L). The inoculum was activated sludge collected from Taylor Creek Wastewater Treatment Plant (Harrison, OH) which receives predominantly domestic sewage. The test treatment and inoculum blank were measured in triplicate, while the reference was measured in duplicate. The deviation between replicate treatments was <20% (met the validity criteria of the guideline). 

No biodegradation of the test material was observed during the 29 days. The final percentage of biodegradation(CO2 evolution) on Day 29 was -4.1% (mean of 3 flasks with -3.6, -7.5 and -1.2%, respectively).

The test item did not meet the criteria for ready biodegradation.

The reference material, sodium benzoate, reached >10% biodegradation at Day 1, and >60% at Day 4 in both replicates. Hence, it met the validity criteria established in the guideline for a reference material.

No toxicity control was performed during this test. So a toxicity effect of the test matrial on the inoculum cannot be ruled out.