Registration Dossier
Registration Dossier
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EC number: 210-236-8 | CAS number: 610-81-1
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
Description of key information
The 7-day ErC50 was calculated to be 10.1 (95% CI: 9.7-10.5) and 8.10 (95% CI: 7.93-8.27) mg test item/L for frond number and dry weight, respectively.
The 7-day ErC10 was calculated to be 3.18 (95% CI: 2.95-3.42) and 2.82 (95% CI: 2.70-2.95) mg test item/L for frond number and dry weight, respectively.
Key value for chemical safety assessment
Additional information
The purpose of this study was to determine the inhibitory effect of the test item on the growth of the freshwater aquatic plant Lemna gibba, according to OECD 221 guideline and following the GLP. For this purpose, cultures of Lemna gibba were exposed in a semi-static test with daily water renewal to various concentrations under defined conditions: 80, 25, 8.0, 2.5 and 0.8 mg test item/L and a control; corresponding to the following geometric mean measured concentrations: 27.8, 8.49, 3.91, 1.26, 0.428 mg test item/L. This study encompassed 6 treatment groups (5 dose rates of the test item and a control) with three replicates per test concentration and control. At test start 12 fronds were introduced in each replicate and incubated for 7 days. The frond numbers were determined on day 2, 5 and 7. The dry weight of each replicate was determined at test termination. The 7-day ErC50 was calculated to be 10.1 and 8.10 mg test item/L for frond number and dry weight, respectively. The 7-day ErC10 was calculated to be 3.18 and 2.82 mg test item/L for frond number and dry weight, respectively. All reported results refer to geometric mean concentrations, since the test item concentrations were not within ± 20% of the nominal concentrations during the test. All the validity criteria were fulfilled accordingto the OECD guideline 221.
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