Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
no analytical follow-up; but the results demonstrate the adequacy of the test system

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no
Remarks:
Total organic carbon follow-up was attempted, but failed due to barely detectable values

Test solutions

Vehicle:
yes
Remarks:
reconstituted water with Ca, Mg, Na, K supplies

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
between 140 and 250 mg CaCO3/L
Test temperature:
20°C +/- 2°C
pH:
8-8.3 at t0
Dissolved oxygen:
> 3mg/L
Nominal and measured concentrations:
nominal: 0, 0.1, 0.17, 0.31, 0.56, 1 mg/L
analysed: not applicable
Reference substance (positive control):
not required

Results and discussion

Effect concentrations
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.155 mg/L
Basis for effect:
mobility
Details on results:
% of immobilized Daphnie in control: 0.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Daphnia magna 48h-EC50 (static assay, mobility) is 0.155 mg/L.