Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: - | CAS number: -
Endpoint summary
Administrative data
Description of key information
Adsorption coefficient
The keystudy was performed to estimate the adsorption coefficient (Koc) of the test item in soil and sludge using a High Performance Liquid Chromatography (HPLC) based methodology. In this method, theretention times of test chemicals are correlated with those for reference standards with known adsorption coefficients. The guidelines applied were OECD Guideline for Testing of Chemicals, 121Estimation of the Adsorption Coefficient (Koc) on Soil and Sewage Sludge using High Performance Liquid Chromatography (HPLC) Method (2001) and Estimation of the Adsorption Coefficient (Koc) on Soil and Sewage Sludge using High Performance Liquid Chromatography (HPLC) Method. Official Journal of the European Communities No. L225, Method C.19 (2001).
Five calibration standards of known log Koc were prepared from reference materials in the respective mobile phase at a nominal concentration range (10.0 to 200 mg/L) selected to provide desired ultraviolet (UV) detector response. Capacity factors were calculated for the five calibration standards using a sixth calibration standard, urea, to estimate the column dead time (i.e. the retention time of an unretained organic compound). The logarithms of the capacity factors were then plotted against published log Koc values for the five calibration standards with known log Koc to establish a linear regression equation.
Test substance solutions were prepared at nominal concentrations of 1.00 mg/mL and 5.00 mg/mL in 55% methanol (MeOH): 45% HPLC-grade reagent water (H2O). The calibration standard preparations were sequentially injected into the HPLC system followed by a single injection of the matrix blank preparation, single injections of each of the three 1.00 mg/mL test substance preparations, and a single injection of the 5.00 mg/mL test substance preparation. The calibration standards injection sequence was repeated following the test substance injections. The HPLC system was operated under standardised isocratic, reverse-phase operating conditions per the guideline.
The test substance eluted as three peaks and the corresponding Log Koc for the test substance by UV ranged from unretained (< urea) to 4.43.
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
