2-anilinoethanol

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

09.01.2018 - 12.01.2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: A052-2017-HEA
- Expiration date of the lot/batch: 17 May 2019.


STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: stored at ambient temperature without exposure to light in a tightly sealed container
- Stability under test conditions: stable
- Water solubility: 50 g/L ± 0.5 g/L at T = 20.0°C +/- 0.5 °C

Analytical monitoring:

yes

Details on sampling:

Four samples from every freshly prepared test item concentration and the control (without algae) were
collected. One series of samples was transferred for chemical determinations at exposure initiation
and the other three series were stored under test conditions and next they were transferred for
chemical analyses daily.
Additionally, samples of pooled replicates (with algae) from every test item concentration plus the
control were collected and transferred for chemical analysis at exposure termination

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Considering the test item’s density, 920 µL of the liquid test item were added into a flask with test
medium using a glass capillary pipette. The contents of the flask were filled up to a total volume of 2 L
with test medium. The resulting test item concentration of 500 mg/L was homogeneous and
transparent and used for exposure. The lower test item concentrations were prepared by sequential dilutions with the test medium. The control was test
medium (without test item).

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata (Reinsch) Korshikov (syn. Raphidocelis subcapitata, Selenastrum capricornutum Prinz) SAG 61.81
- Source (laboratory, culture collection): Unicellular freshwater green algae Pseudokirchneriella subcapitata (Reinsch) Korshikov (syn. Selenastrum capricornutum Prinz, Raphidocelis
subcapitata Korshikov), specification SAG 61.81, cultured in the Institute of Industrial Organic Chemistry, Branch Pszczyna, Department of Ecotoxicology, Laboratory of Aquatic Toxicology, stock from the Culture Collection of Algae at Göttingen University, Germany.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Hardness:

20.3 mg/L as CaCO3

Test temperature:

21.8 – 22.2ºC

pH:

7.52 – 8.63

Nominal and measured concentrations:

Nominal: 500; 250; 125; 62.5; 31.3; 15.63; 7.81 and 3.91 mg/L plus the control
Measured: 485.0; 244.2; 104.1; 41.8; 17.7; 7.68; 4.43; 2.00; 0.00 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: The glass Erlenmeyer flasks with a capacity of 250 mL containing 100 mL of either the test item
concentration or the control plugged with air permeable stoppers were used as test vessels.
- Initial cells density: 1 x 104 cells per mL.
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6


GROWTH MEDIUM
- Standard medium used: yes


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The AAP medium (U.S. EPA) recommended by OECD Guideline No. 201 (2006) [1] was used as a culture medium for the test organism and a diluent/solvent of the test item.

OTHER TEST CONDITIONS

- Light intensity and quality: 5990 – 6800 lux; constant illumination


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: was determined by counting the number of cells in the Bürker chamber under a microscope.

TEST CONCENTRATIONS

- Test concentrations: was determined on the basis of the preliminary (non-GLP) test results

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

254.6 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

109.9 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Results with reference substance (positive control):

- Results with reference substance valid? yes
- EC50: 3.23 mg/L

Validity criteria fulfilled:

yes

Description of key information

With high propability, acutely not toxic to algae.

Key value for chemical safety assessment

EC50 for freshwater algae:

254.6 mg/L

EC10 or NOEC for freshwater algae:

109.9 mg/L

Additional information

Toxicity of 2 -Aminoethanol was tested in a study follwoing OECD 201 in a static system (study number 60E0452/14X656). The substance was tested in concentrations of 500; 250; 125; 62.5; 31.3; 15.63; 7.81 and 3.91 mg/L plus the controls. The sudy was accompanied by analytical verivication of the test concentrations. In controls with test substance but without addition of algae, the test item concentrations remained within +/-20% of nominal concentrations. However, the analytically verified concentrations in the test samples with alge varied more than 20% at the end of exposure compared to the initial concentration. Therefore, the final results of the study are experessed based on the arithemtic mean of the single test sample concentrations. The ErC50 turned out at 254 mg/L and the ErC10 was determined at 109.9 mg/L.