Reaction product of di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid and alkanolamine

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Remarks:

the tested substance represents the cationic part of the salt

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions (e.g. non-GLP, no analytical verification of test concentrations)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: 79/831/EEC, C.2

GLP compliance:

no

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): Di-isopropanolamine- Analytical purity: no data

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM- Source: Institut National de Recherche,; bred since 1978 at Laboratory for Environmental Analysis and Ecology   - Age at study initiation (mean and range, SD): 2-24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.55 mmol/l  

Test temperature:

19-21 °C

pH:

7.7-8.3

Nominal and measured concentrations:

Nominal test concentrations: 0 (control), 7.81, 15.6, 31.2, 62.5, 125, 250, and 500 mg/l

Details on test conditions:

TEST SYSTEM- Test vessel: glass tubes with flat bottom - Fill volume: 10 ml (2 ml/animal) - Aeration: aerated till saturation with oil-free air and allowed to stabilize for 24 h- No. of organisms per vessel: 5- No. of vessels per concentration (replicates): 4- No. of vessels per control (replicates): 4TEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: artificial freshwater: chlorine-free filtered tapwater supplemented with H2SO4 for the reduction of the buffering capacity of the carbonic acid system, and with deionized water for the reduction of the total hardness. Total hardness 2.70 +- 0.5 mmol/l, acid capacity to pH 4.3 0.80 +- 0.10 mmol/l, ratio of Na:K ions 10:1, - Ca/mg ratio: 4:1- Conductivity: 550-650 µSiemens/cmOTHER TEST CONDITIONS- Light intensity: diffuse lightEFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mobility observation times: visually after 0, 3, 6, 24 and 48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

125 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

277.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 239.4 - 322.11 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

EC 50 after 24 h and EC 50 after 48 h were determined according to Spearman-Kaerber (Sachs, L. (1974): Angewandte Statistik, Springer, Berlin.)

Any other information on results incl. tables

Observed immobility:

Nominal conc. (mg/l)	cumulative immobility (n)
	0 h	3 h	6 h	24 h	48 h
0 (Control)	0	0	0	0	0
7.81	0	0	0	0	0
15.6	0	0	0	0	0
31.2	0	0	0	0	0
62.5	0	0	0	0	0
125	0	0	0	0	0
250	0	0	0	0	7
500	0	0	3	20	20

Measured data at the beginning of the test concerning test medium:

Measured pH and oxygen values:

Nominal conc.(mg/l)	pH		oxygen (mg/l)
	0 h	48 h	0 h	48 h
0 (Control)	8.0	7.9	8.8	8.3
7.81	8.3	8.0	8.6	8.7
15.6	8.6	8.0	8.6	8.6
31.2	8.9	8.0	8.6	8.5
62.5	9.1	8.2	8.4	8.5
125	9.3	8.5	8.4	8.3
250	9.5	8.8	8.5	8.3
500	9.7	9.1	8.5	8.3