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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Remarks:
the tested substance is the amine component of the compound
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restriction (e.g. non-GLP)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report Date:
1995

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Di-isopropanolamine- Analytical purity: 99.3 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Method: The product was added to the test water without any pretreatment; subsequently the fish were placed into the aquaria after 20 min.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM- Common name: Zebra fish- Age at study initiation (mean and range, SD): ca. 3.5 months- Length at study initiation (length definition, mean, range and SD): 3.29 cm (range: 2.8-3.6 cm)- Weight at study initiation (mean and range, SD): 0.305 g (range: 0.16-0.45 g- Method of breeding: - Feeding during test: 1 day before and during exposureACCLIMATION- Acclimation period:- Acclimation conditions (same as test or not): test conditions correspond to housing conditions- Type and amount of food:- Feeding frequency: - Health during acclimation (any mortality observed):QUARANTINE (wild caught)- Duration: - Health/mortality:

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
2.5 mmol/l (ca. 250 mg/l CaCO3)
Test temperature:
21-23 °C (average: 22.6 °C).
Nominal and measured concentrations:
Nominal test concentrations: 0 (control), 46.4, 100, 215, 464, 1000, 2150, and 10000 mg/l; additional test concentrations with pH adjustment: 1000 and 10000 mg/lConcentration control analysis (recovery: 95-106 %)
Details on test conditions:
TEST SYSTEM- Test vessel: : non-sealed all-glass aquaria (30x22x24 cm) - Fill volume: 10 l (water depth: ca. 17 cm) - Aeration: continuous and slight aeration with oil-free air - No. of organisms per vessel: 10- No. of vessels per concentration (replicates): 1- No. of vessels per control (replicates): 1- Biomass loading rate: 0.3 g fish / l test waterTEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: municipal freshwater (Frankenthal, Germany), not chlorinated, passed through an active carbon filter. Total hardness: acid capacity: 5.5 mmol/l, Ca: ca. 90 mg/l, Mg: ca. 10 mg/l, pH ca. 8.0-8.6- Intervals of water quality measurement: temperature, pH, and oxygen content after 1, 24, 48, 72, and 96 hOTHER TEST CONDITIONS- Photoperiod: 16 h light and 8 h darknessEFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality and symptoms after 1, 4, 24, 48, 72, and 96 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
464 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1 466 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: ; geometrical mean value
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
2 150 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
White sediment was visible on the bottom of the aquaria.To study the effect of the relatively high pH on the toxicity, the concentrations 1000 and 10000 mg/l were tested in parallel after pH adjustment. At 1000 mg/l (nominal, neutralized) no dead fish where observed after 96 h while at 10000 mg/l (nominal; neutralized) all fish died.
Reported statistics and error estimates:
data evaluation: the number of dosis tested did not allow for a Probit analysis to be performed, since the data did not yield intermittent values between the LC0 (ca. 1000 mg/l) and the LC100 (ca. 2150 mg/l). Therefore the geoemtric mean of the two concentrations was calculated: LC50 (96 h): 1466 mg/l (nominal, based on measured data)

Any other information on results incl. tables




Nominal concentration [mg/l]

Cumulative mortality (n)

 

1 h

4 h

24 h

48 h

72 h

96 h

Control

0

0

0

0

0

0

Control

0

0

0

0

0

0

46.4

0

0

0

0

0

0

100

0

0

0

0

0

0

215

0

0

0

0

0

0

464

0

0

0

0

0

0

1000x

0

0

0

0

0

0

1000x

0

0

0

0

0

0

2150

0

0

9

10

10

10

4640

6

10

10

10

10

10

10000

10

10

10

10

10

10

1000*

0

0

0

0

0

0

10000*

1

9

10

10

10

10

x: performed in duplicate
*: pH adjusted


Nominal concentration [mg/l]

Symptoms (n)

 

1 h

4 h

24 h

48 h

72 h

96 h

Control

-

-

-

-

-

-

Control

-

-

-

-

-

-

46.4

-

-

-

-

-

-

100

-

-

-

-

-

-

215

-

-

-

-

-

-

464

-

-

-

-

-

-

1000x

-

-

-

-

-

-

1000x

A

A

-

-

-

-

2150

A

AT

A

x

x

x

4640

AT

x

x

x

x

x

10000

x

x

x

x

x

x

1000*

-

-

-

-

-

-

10000*

A

A

x

x

x

x

Legend: A = apathy; T = tumbling



Nominal concentration [mg/l]

Oxygen concentration [mg/l]

 

1 h

4 h

24 h

48 h

72 h

96 h

Control

7.9

7.9

7.8

7.8

7.7

 

Control

8.1

7.9

7.3

7.4

7.7

 

46.4

8.1

7.9

7.9

8.2

7.6

 

100

8.1

8.0

7.9

8.2

7.8

 

215

8.0

7.9

7.7

8.1

7.7

 

464

8.1

7.9

7.7

8.2

7.7

 

1000x

8.1

8.2

8.1

8.4

8.0

 

1000x

7.9

8.0

7.5

7.4

8.0

 

2150

8.0

8.2

7.7

 

 

 

4640

8.1

 

 

 

 

 

10000

8.1

 

 

 

 

 

1000*

8.0

7.7

7.8

8.2

7.8

 

10000*

8.0

8.3

 

 

 

 

*: pH adjusted



Nominal concentration [mg/l]

pH

 

1 h

4 h

24 h

48 h

72 h

96 h

Control

8.4

8.3

8.3

8.4

8.4

 

Control

8.5

8.4

8.4

8.4

8.4

 

46.4

8.5

8.3

8.3

8.4

8.4

 

100

8.7

8.4

8.4

8.4

8.4

 

215

8.8

8.4

8.4

8.4

8.4

 

464

9.0

8.8

8.7

8.7

8.7

 

1000x

9.3

9.1

9.0

9.0

8.9

 

1000x

9.3

9.2

9.2

9.2

9.1

 

2150

9.5

9.5

9.4

 

 

 

4640

9.7

 

 

 

 

 

10000

10.0

 

 

 

 

 

1000*

8.6

8.4

8.3

8.4

 

 

10000*

8.7

8.7

 

 

 

 



Test item concentrations:

Nominal concentration [mg/l]

analytically detected concentrations
(% of nominal)

 

1 h

96 h

Control

+

+

Control

 

 

46.4

99.1

105.6

100

98.0

101.0

215

95.3

98.1

464

95.9

98.5

1000x

95.6

98.2

1000x

 

 

2150

 

 

4640

 

 

10000

 

 

1000*

95.9

99.2

10000*

 

 


+: not detectable

 

Applicant's summary and conclusion