Registration Dossier

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Principles of method if other than guideline:
Oxygen Consumption Test (Huels Method). Oxygen concentration of controls and test replicates are compared after 4 - 6 hours of incubation.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
4-tert-butyltoluene
EC Number:
202-675-9
EC Name:
4-tert-butyltoluene
Cas Number:
98-51-1
Molecular formula:
C11H16
IUPAC Name:
1-tert-butyl-4-methylbenzene
Details on test material:
- Name of test material (as cited in study report): p-tert-butyltoluene
- Analytical purity: 96.3 %
- Lot/batch No.:none, Reaktor C510

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by eThe test substance was emulsified in Nonylphenol (10 EO, 5 PO)

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
- Laboratory culture: obtained from Dr. Reinhard Kanne, Bayer AG, Leverkusen, Germany

Study design

Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
4.58 h

Test conditions

Test temperature:
24.7 - 25.3 °C
Nominal and measured concentrations:
Nominal test concentrations:
1, 10, 100 and 1000 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: glass flask (100 ml)
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

Results and discussion

Effect concentrationsopen allclose all
Duration:
5 h
Dose descriptor:
EC0
Effect conc.:
>= 1 000 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
5 h
Dose descriptor:
EC10
Effect conc.:
> 1 000 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
5 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Applicant's summary and conclusion