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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Directive 92/69/EEC
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
4-tert-butyltoluene
EC Number:
202-675-9
EC Name:
4-tert-butyltoluene
Cas Number:
98-51-1
Molecular formula:
C11H16
IUPAC Name:
1-tert-butyl-4-methylbenzene
Details on test material:
- Name of test material (as cited in study report): p-tert-butyltoluene
- Analytical purity: 96.3 %
- Lot/batch No.: no batch No., Reaktor C 510

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1g/l of the test substance was dissolved in synthetic test water and stirred for 18 hours. Subsequently the solution was filtered and the DOC was measured. DOC: 6.3 mg/l

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Age at study initiation (mean and range, SD): < 24 h

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
20 ± 1 °C
Nominal and measured concentrations:
Nominal test concentrations:
control, 0.7, 1.2, 2.1, 3.5 and 6.3 mg/l

Measured test concentrations after 0 h:
control, 1.2, 1.6, 2.3, 3.7 and 6.6 mg/l

Measured test concentrations after 48 h:
control, 0.44, 0.75, 1.0, 1.6 and 2.7 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: glass vessels (10 ml)
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates):


OTHER TEST CONDITIONS
- Photoperiod: continous darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
mobility after 0 ,24 and 48 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
2.4 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
3.2 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 4.2 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion