Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 220-474-4
CAS number: 2778-42-9
of mortality data
LD50 (mg/kg bw) = LD50 (mL/kg bw) X Density
where, density of the test material =
1.06749 g/cm3 = 1067.49 mg/mL
study was conducted to determine the acute oral toxicity of the test
substance in Sprague-Dawley rats. The study was performed according to a
method similar or equivalent to OECD Guideline 401 in compliance with
GLP. Test substance was administered orally (gavage) to 5
animals/sex/dose at 0, 2.8, 3.6, 4.5, 5.6 and 7.1 mL/kg. Body weights
were obtained on Days-1, 1 (Day of dosing), 2, 3, 4, 7, 11 and 15 or at
time of death if prior to Day 15. Animals were frequently observed for
clinical signs and mortality on the day of dosing and twice daily
thereafter for 14 days. Animals found dead and all those surviving for
the duration of the study were subjected to gross necropsy. Treated
animals gained less weight than untreated animals and lost weight for 1
to 3 days after test substance administration. Clinical signs noted were
lethargy, diarrhoea, urine-soaked fur and lacrimation. These signs may
have negatively affected the intake of food, resulting in reduced body
weight. Clinical signs and gross necropsy findings were seen throughout
all dose levels and did not indicate any sex or dose related trends.
Under the test conditions, the LD50 of the test substance were
determined to be 5.0 (4.4 - 5.7) and 4.6 (2.5 -8.4) mL/kg for males and
females, respectively. The combined LD50 of the test substance was
calculated to be 5.0 (4.0-6.2) mL/kg [equivalent to 5337 (4270-6618)
mg/kg bw] (Calkins, 1981).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again