Lavandula hybrida oil, light fraction

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

other: bibliography

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data comes from an online compilation using secondary data sources. There is no experimental study reference, however data is cited as official publication.

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

GLP compliance:

no

Specific details on test material used for the study:

Linalyl acetate, purity 96.0%.

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Activated sludge (30 mg/L): prepared from soil of river bank, sludge of a municipal treatment plant and sludge of an industrial sewage plant.
- 2 flasks Treated (basal culture medium + activated sludge + linalyl acetate (100-102 mg/L));
- 1 flask Positive Control (basal culture medium + activated sludge + Aniline (92 mg/L));
- 1 flask Blank Control (basal culture medium + activated sludge).

Duration of test (contact time):

28 d

Parameter:

% degradation (O2 consumption)

Value:

> 75

Sampling time:

28 d

Validity criteria fulfilled:

not specified

Interpretation of results:

readily biodegradable

Conclusions:

Under the test conditions, linalyle acetate is readily biodegradable.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

other: bibliography

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data comes from an online compilation using secondary data sources. There is no experimental study reference, however data is cited in the database as peer reviewed.

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Principles of method if other than guideline:

source : NITE; Chemical Risk Information Platform (CHRIP). Biodegradation and Bioconcentration. Tokyo, Japan: Natl Inst Tech Eval. Available from, as of May 21, 2014: http://www.safe.nite.go.jp/english/db.html **PEER REVIEWED**

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

at 30 mg/L

Duration of test (contact time):

4 wk

Details on study design:

Camphor, present at 100 mg/L, reached 94% of its theoretical BOD in 4 weeks using an activated sludge inoculum at 30 mg/L in the Japanese MITI test which classified the compound as readily biodegradable.

Parameter:

% degradation (O2 consumption)

Value:

94

Sampling time:

4 wk

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Camphor, present at 100 mg/L, reached 94% of its theoretical BOD in 4 weeks using an activated sludge inoculum at 30 mg/L in the Japanese MITI test which classified the compound as readily biodegradable(4).

Reference 3

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

other: bibliography

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data comes from an online compilation using secondary data sources. There is no experimental study reference, however data is cited in the database as peer reviewed.

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Principles of method if other than guideline:

source : NITE; Chemical Risk Information Platform (CHRIP). Biodegradation and Bioconcentration. Tokyo, Japan: Natl Inst Tech Eval. Available from, as of Apr 24, 2015: http://www.safe.nite.go.jp/english/db.html (5) Misra G et al; Appl Microbiol Biotechnol
45: 831-8 (1996)] **PEER REVIEWED**

GLP compliance:

not specified

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Limonene, present at 100 mg/L, reached 73% of its theoretical BOD in 2 weeks using an activated sludge inoculum at 30 mg/L in the Japanese MITI test

Parameter:

% degradation (O2 consumption)

Value:

ca. 73

Sampling time:

14 d

Validity criteria fulfilled:

not specified

Interpretation of results:

readily biodegradable

Conclusions:

Under the test conditions, Limonene was readily biodegradable.

Reference 4

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

other: bibliography

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data comes from an online compilation using secondary data sources. There is no experimental study reference, however data is cited in the database as peer reviewed.

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Principles of method if other than guideline:

(1) NITE; Chemical Risk Information Platform (CHRIP). Biodegradation and Bioconcentration. Tokyo, Japan: Natl Inst Tech Eval. Available from, as of Nov 20, 2015: http://www.safe.nite.go.jp/english/db.html
(2) ECHA; Search for Chemicals. Linalool (CAS 78-70-6) Registered Substances Dossier. European Chemical Agency. Available from, as of Nov 20, 2015: http://echa.europa.eu/
(3) OECD; SIDS Initial Assessment Report For SIAM 14, Linalool (CAS No. 78-70-6), March 2002. Available from, as of Nov 20, 2015: http://www.chem.unep.ch/irptc/sids/OECDSIDS/78706.pdf **PEER REVIEWED**

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Parameter:

% degradation (O2 consumption)

Value:

90

Sampling time:

4 wk

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Linalool, present at 100 mg/L, reached 90% of its theoretical BOD in 4 weeks using an activated sludge inoculum at 30 mg/L in the Japanese MITI test which classified the compound as readily biodegradable

Description of key information

86% of the UVCB substance is composed by readily biodegradable constituents (see table below):

Constituents	CAS No	% in the UVCB	Biodegradation results	source
Linalool	78-70-6	64%	Readily biodegradable	TOXNET / HSDB
Linalyl acetate	115-95-7	1%	Readily biodegradable	OECD SIDS - UNEP publication
Camphor	76-22-2	13%	Readily biodegradable	TOXNET / HSDB
beta-ocimene	13877 -91 -3	7%	Readily biodegradable	ECHA / REACH dossier
Limonene	138 -86 -3	1%	Readily biodegradable	TOXNET / HSDB

Based on a Weight of Evidence approach, it can be possible to consider that LAVANDIN OIL light fraction is readily biodegradable.