Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 402-010-4 | CAS number: 4463-59-6
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin corrosion: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 431 (In Vitro Skin Corrosion: Reconstructed Human Epidermis (RHE) Test Method)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1-(2-bromoethoxy)-2-methoxybenzene
- EC Number:
- 402-010-4
- EC Name:
- 1-(2-bromoethoxy)-2-methoxybenzene
- Cas Number:
- 4463-59-6
- Molecular formula:
- C9H11BrO2
- IUPAC Name:
- 1-(2-bromoethoxy)-2-methoxybenzene
- Details on test material:
- - Name of test material (as cited in study report): Carvedilol 5th intermediate (Bromoethoxy-guaiacol)
- Physical state: reddish brown crystalline powder
- Lot/batch No.: 9803
- Expiration date of the lot/batch: June 2001
- Storage condition of test material: at room temperature
Constituent 1
In vitro test system
- Test system:
- human skin model
- Source species:
- human
- Cell type:
- non-transformed keratinocytes
- Vehicle:
- unchanged (no vehicle)
- Details on test system:
- SKIN DISC PREPARATION
- Procedure used: EpiSkin SM kit
- Quality control for skin discs: certified ISO 9001
TEMPERATURE USED FOR TEST SYSTEM
- Temperature used during treatment- exposure: room temperature
DYE BINDING METHOD
- Dye used in the dye-binding assay: MTT
- Spectrophotometer: 96-well plate spectrophotometer
- Wavelength: 570 nm - Amount/concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 20 mg
NEGATIVE CONTROL
- Amount(s) applied (volume or weight): 50 mikroliter
- Concentration (if solution): 100%
POSITIVE CONTROL
- Amount(s) applied (volume or weight): 50 mikroliter
- Concentration (if solution): 100 % - Duration of treatment / exposure:
- 4 hours
- Number of replicates:
- 2
Results and discussion
In vitro
Results
- Irritation / corrosion parameter:
- % tissue viability
- Run / experiment:
- mean
- Value:
- 86
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: no skin corrosion potential
Applicant's summary and conclusion
- Interpretation of results:
- other: Non-corrosive
- Conclusions:
- The results obtained from this in vitro skin corrosion test, using the EPISKIN model, indicated that the test item reveals no skin corrosion potential under the utilised testing conditions. In conclusion, the test item Bromethoxy-qvajacol (CVD 21) can be classified as Non-corrosive.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
