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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report Date:
1990

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
Preparation with stock solution of 10 mg/L and dilution water

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: Strain optained from BGA, Berlin

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
20.1 - 21.0°C
Dissolved oxygen:
8.2 - 8.7 mg/L
Nominal and measured concentrations:
Nominal: 0 (control>), 0.091, 0.16, 0.53, 0.95 and 1.7 mg/L
Details on test conditions:
Water quality parameters according to guideline
Dilution water / test medium: Elendt M4
Replicates (test concentration, negative and positive controls): 3
Loading: according to guideline
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
0.16 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
0.29 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.22 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: geometric mean EC0/EC100
Results with reference substance (positive control):
- Dose-response test: concentrations 0.5, 1.0, 1.5, 2.0, 3.0, 4.0 and 5.0 mg/L
- EC50 (24h) 2.4 mg/L (Probit analysis)

Any other information on results incl. tables

concentration
[mg/L]
cumulative immobilisation rate
[%]
24h 48h
control 0 0
0.09 0 0
0.16 0 0
0.29 45 95
0.53 75 100
0.95 90 100
1.7 100 100

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48h- EC50 to Daphnia magna was calculated to be 0.22 mg/L. The substance is acutely toxic to aquatic invertebrates.