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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2017-02-06 - 2017-03-06
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report Date:
2017

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Version / remarks:
2006-03-23;
Annex 5 corrected 2011-07-28
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
impurity
Type:
impurity
Test material form:
solid: bulk
Details on test material:
This composition is the usual techical grade of AlzChem AG. It will be used when test substance has this composition (or is very closed) or no specific information is available

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:
static
Water media type:
other: mineral medium (AAP-Medium)
Remarks:
See Annex 3 of OECD 201 "Freshwater Alga and Cyanobacteria, Growth Inhibition Test", 2011
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
22.3 - 23.0 °C
pH:
7.5 +/-0.1

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
ca. 0.583 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
ca. 5.51 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
ca. 1.23 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Key result
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
ca. 0.477 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
not specified
Remarks on result:
other: Bonferroni-Holms corrected Welch test for growth rate
Remarks:
Dunnetts-t-test for yield (left sided, p < 0.5))
Duration:
72 h
Dose descriptor:
NOEC
Remarks on result:
not measured/tested

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Significant inhibitory effects were determined for growth rate and for yield at test item
concentrations of 0.477 mg/L (nominal) and 0.0698 mg/L (actual) and above. The overall LOEC
was therefore determined to be 0.477 mg/L (nominal) and 0.0698 mg/L (actual), the corresponding
NOEC could not be determined.
The EC10-value for growth rate (ErC10) was determined to be 0.583 mg/L (nominal). The EC20-value
was determined to be 1.34 mg/L (nominal) and 0.213 mg/L (actual). The EC50-value for growth rate
(ErC50) was determined to be 5.51 mg/L (nominal) and 1.67 mg/L (actual). The EC50-value for yield
(EyC50) was 1.23 mg/L (nominal) and 0.186 mg/L (actual).
Since the estimated EC10 and EC20 values for yield (actual and nominal) and the EC10 for growth rate
(actual) was below the lowest test item concentration, these values are not reported.