1,3,3-trimethyl-2-[[methyl(p-tolyl)hydrazono]methyl]-3H-indolium methyl sulphate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995-01-16 to 1995-02-13

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-[[(4-methoxyphenyl)methylhydrazono]methyl]-1,3,3-trimethyl-3H-indolium methyl sulphate

EC Number:

258-946-7

EC Name:

2-[[(4-methoxyphenyl)methylhydrazono]methyl]-1,3,3-trimethyl-3H-indolium methyl sulphate

Cas Number:

54060-92-3

Molecular formula:

C20H24N3O.CH3O4S C21H27N3O5S

IUPAC Name:

2-{[2-(4-methoxyphenyl)-2-methylhydrazin-1-ylidene]methyl}-1,3,3-trimethyl-3H-indol-1-ium methyl sulfate

Test material form:

solid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

other: see below

Details on inoculum:

- Test organisms: aquatic mix-population of activated sludge
- Source of inoculum:
Activated sludge I: municipal ("Kläranlage im Labormaßstab, die mit kommunalen Abwasser betrieben wird (Wupperverband)")
Activated sludge II: predominantly municipal ("Kläranlage Odenthal, wird vorwiegend mit kommunalem Abwasser betrieben.")
Activated sludge III: predominantly industrial ("Belebungsbecken des Gemeinschaftsklärwerks Bürrig wird vorwiegend mit industriellem Abwasser betrieben.")
- Pretreatment: each sludge was washed twice, and separated via centrifugation

Duration of test (contact time):

ca. 28 d

Initial test substance concentration

Initial conc.:

ca. 100 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (DOC removal)

Value:

ca. 1

Sampling time:

28 d

Remarks on result:

other: Activated sludge I - municipal

% Degradation 2

Parameter:

% degradation (DOC removal)

Value:

ca. 2

Sampling time:

28 d

Remarks on result:

other: Activated sludge II - predominantly municipal

% Degradation 3

Parameter:

% degradation (DOC removal)

Value:

ca. 6

Sampling time:

28 d

Remarks on result:

other: Activated sludge III - predominantly industrial

BOD5 / COD results

Results with reference substance:

100% biodegradation after 14 days with each sludge type.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not inherently biodegradable

Conclusions:

The test substance was tested for inherent biodegradation in a modified Zahn-Wellens test according to OECD 302B. The DOC-removal with three different sludge types was consistently below 10% after 28 days clearly showing that the test substance has to be considered as "not inherently biodegradable".

Executive summary:

The test substance was tested for inherent biodegradation in a modified Zahn-Wellens test according to OECD 302B. A significant biological degradation was not noted. The DOC-removal with three different sludge types was consistently below 10% after 28 days.