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EC number: 238-339-3 | CAS number: 14367-46-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- of read across substance
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on two short term toxicity study of fish for the test chemical :
1.Determination of the inhibition of the mobility of Daphnids was carried out with the test chemical according to OECD Guideline 202.
2. EC50 value of test material was measured on crustacean Daphnia magna for 48 hrs. - GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (IUPAC name): 2-(N-ethylamino)-1-(4-methoxyphenil propane)
- IUPAC name: 2-(N-ethylamino)-1-(4-methoxyphenil propane)
- Molecular formula: C12H19NO
- Molecular weight: 193.2881 g/mol
- Smiles notation: CCNC(C)Cc1ccc(OC)cc1
- InChl: 1S/C12H19NO/c1-4-13-10(2)9-11-5-7-12(14-3)8-6-11/h5-8,10,13H,4,9H2,1-3H3
- Substance type: Organic - Analytical monitoring:
- not specified
- Details on sampling:
- 2.- Method of confirmation of identity of measured compound: UV–visible spectrophotometer, UV mini 1240
- Vehicle:
- not specified
- Details on test solutions:
- 1. PREPARATION AND APPLICATION OF TEST SOLUTION
The stock solution 150.0 mg/L was prepared by dissolving white powder in reconstituted water. The solution was kept in ultrasonic bath for 20 min. The test solutions of required concentrations were prpared by mixing the stock solution of the test sample in reconstituted water.
- Controls: Reconstituted water as specified in OECD 202, used as a control and for sample dilution. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- 1.TEST ORGANISM
- Common name: Water flea
- Strain/clone: Straus
- Source: Own breeding at University of Chemistry and Technology, Prague
- Age: Young daphnids, aged less than 24 hours used at the start of the test and they should not be first brood progeny.
- Feeding during test : Without feeding
ACCLIMATION
- Acclimation period: No data
- Acclimation conditions (same as test or not): No data
- Type and amount of food: No data
- Feeding frequency: No data
- Health during acclimation (any mortality observed): No data
QUARANTINE (wild caught)
- Duration: No data
- Health/mortality: No data
2.- Common name: water flea
- Source: National Institute for Environmental Studies (NIES), Tsukuba, Japan
- Age at study initiation (mean and range, SD):<24 hrs (neonates) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 2.~100 mg/l as CaCO3
- Test temperature:
- 1) 20 °C ± 1 °C
- pH:
- 1. Sample at concentration 150 mg/L: pH = 7.3 changed to pH = 7.2 during the test, Control: pH = 7.8 changed to pH = 7.6 during the test.
2. 7.45 ± 0.05 - Dissolved oxygen:
- 1.Higher than 8.1 mg/L both in the control and the sample.
- Nominal and measured concentrations:
- 1) 0, 30.0, 45.0, 67.5, 100.0 and 150.0 mg/L.
- Details on test conditions:
- 1.TEST SYSTEM
- Test vessel: 50 ml glass vessel
- Material, size, headspace, fill volume: 25 ml
- Aeration: no data
- No. of organisms per vessel: 5 pieces
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): No data
- No. of vessels per vehicle control (replicates): No data
- Biomass loading rate: No data
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural water (surface or ground water), reconstituted water or dechlorinated tap water are acceptable as culturing and dilution water if D. magna survives in it for the duration of the culturing, acclimation and testing without showing signs of stress. Waters in the range pH 6 to pH 9, with hardness between 140 mg/l and 275 mg/l (as CaCO3) are recommended.As an example, the preparation of dilution water meeting the requirements is described below.Dissolve known quantities of reagents in water. The dilution water prepared shall have a pH of 7.8 ± 0.5, a hardness of (225 ± 50) mg/l (expressed as CaCO3), a molar Ca + Mg ratio close to 4 + 1 and a dissolved oxygen concentration above 7 mg/l.
Prepare the solutions specified below:
- Calcium chloride solution: Dissolve 117.6 g of calcium chloride dihydrate (CaCl2.2H2O) in water (4.2) and make up to 1 l with water (4.2).
- Magnesium sulfate solution: Dissolve 49.3 g of magnesium sulfate heptahydrate (MgSO4.7H2O) in water (4.2) and make up to 1 l with water (4.2).
- Sodium bicarbonate solution: Dissolve 25.9 g of sodium bicarbonate (NaHCO3) in water (4.2) and make up to 1 l with water (4.2).
- Potassium chloride solution: Dissolve 2.3 g of potassium chloride (KCI) in water (4.2) and make up to 1 l with water (4.2).
Mixing
Mix 2.5 ml of each of the four solutions and make up to 1 l with water.
The dilution water shall be aerated until the dissolved oxygen concentration has reached saturation and the pH has stabilized. If necessary, adjust the pH to 7.8 ± 0.5 by adding sodium hydroxide (NaOH) solution or hydrochloric acid (HCI). The dilution water prepared in this way shall not be further aerated before use.
- Sodium hydroxide solution, e.g. [NaOH] : 1 mol/l.
- Hydrochloric acid, e.g. [HCl] : 1 mol/l.
OTHER TEST CONDITIONS
- Adjustment of pH: Without adjustment
- Photoperiod: No - Darkness
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : EC50 was calculated using non linear regression by the software Prism 4.0.
2. Test vessel: 50 ml Beaker
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 40ml
- Aeration: before the test start
- Renewal rate of test solution (frequency/flow rate): The medium was renewed three times a week ( during culture condition)
- No. of vessels per concentration (replicates): 4 replicates
OTHER TEST CONDITIONS
- Adjustment of pH: Yes with the help of NaOH and HCL
- Photoperiod: 16 h light: 8 h dark photoperiod
TEST CONCENTRATIONS
- Test concentrations: 851.984 -1019.338 mg/l - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7) for read across 1
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 100.8 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Read across 1
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 943.26 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Read across 2
- Results with reference substance (positive control):
- 1.- Results with reference substance valid
- 24h EC50 = 0.73 mg/L K2Cr2O7 - Validity criteria fulfilled:
- not specified
- Conclusions:
- The test chemical 4-Methoxybenzylamine is not likely to be toxic to Daphnia magna atleast in the dose range of 100.8-943.26 mg/l
- Executive summary:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity to aquatic invertebrates of the test chemical 2-(N-ethylamino)-1-(4-methoxyphenil propane) (14367 -46 -5).The studies are as mentioned below:
1.Determination of the inhibition of the mobility of Daphnids was carried out with the test materialaccording to OECD Guideline 202.
The stock solution 150.0 mg/L was prepared by dissolving white powder in reconstituted water. The solution was kept in ultrasonic bath for 20 min. The test solutions of required concentrations were prpared by mixing the stock solution of the test sample in reconstituted water.The test substance was tested at the concentrations 0, 30.0, 45.0, 67.5, 100.0 and 150.0 mg/L.The test was performed under static conditions in a fresh water system at a temperature of 20 °C± 1 °C. EC50 was calculated using non linear regression by the software Prism 4.0.
The median effective concentration (EC50) for the test substance, test material, in Daphnia magna was determined to be 100.8 mg/L for immobilisation effects with 95% CI of 94.7 to 107.4 mg/L.
2.Short term toxicity to aquatic invertebrates was performed in Daphnia magna for 48 hrs. The young daphnids were obtained from continuous cultures in 1 liter glass beakers at 21 ± 0.3 degree C, in dechlorinated and conditioned tap water, the medium was renewed in three weeks and daphnids were fed daily with the green alga Selenastrum capricornutum. The pH of test solution was adjusted to 7.45 ± 0.05 with HCL and NaOH. The concentrations of the resultant solutions were checked before and after (48 h) exposure using a UV–visible spectrophotometer.
As the experiment starts neonate were placed in 50 ml of beaker with 40 ml of test solution. All experiment was made in four replicates and performed at 21 ± 0.3 degree C under 16 h light: 8 h dark photoperiod.
Immobility was observed after 24 and 48 hrs and EC 50 value was calculated. After the experiment the EC 50 value of test material for Daphnia magna was determined to be 943 mg/l in 48 hrs.
Thus, based on the above summarised studies, 2-(N-ethylamino)-1-(4-methoxyphenil propane) and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range 100.8 to 943.26 mg/L. Thus, comparing this value with the criteria of CLP regulation , 2-(N-ethylamino)-1-(4-methoxyphenil propane) cannot be classified for short term toxicity to aquatic invertebrates .Hence,based on the data available for the structurally and functionally similar read across, test chemical 2-(N-ethylamino)-1-(4-methoxyphenil propane) is not likely to be toxic atleast in the concentration range of 100.8 to 943.26 mg/L
.
Reference
Description of key information
Short-term toxicity to aquatic invertebrates:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity to aquatic invertebrates of the test chemical 2-(N-ethylamino)-1-(4-methoxyphenil propane) (14367 -46 -5).The studies are as mentioned below:
1.Determination of the inhibition of the mobility of Daphnids was carried out with the test materialaccording to OECD Guideline 202.
The stock solution 150.0 mg/L was prepared by dissolving white powder in reconstituted water. The solution was kept in ultrasonic bath for 20 min. The test solutions of required concentrations were prpared by mixing the stock solution of the test sample in reconstituted water.The test substance was tested at the concentrations 0, 30.0, 45.0, 67.5, 100.0 and 150.0 mg/L.The test was performed under static conditions in a fresh water system at a temperature of 20 °C± 1 °C. EC50 was calculated using non linear regression by the software Prism 4.0.
The median effective concentration (EC50) for the test substance, test material, in Daphnia magna was determined to be 100.8 mg/L for immobilisation effects with 95% CI of 94.7 to 107.4 mg/L.
2.Short term toxicity to aquatic invertebrates was performed in Daphnia magna for 48 hrs. The young daphnids were obtained from continuous cultures in 1 liter glass beakers at 21 ± 0.3 degree C, in dechlorinated and conditioned tap water, the medium was renewed in three weeks and daphnids were fed daily with the green alga Selenastrum capricornutum. The pH of test solution was adjusted to 7.45 ± 0.05 with HCL and NaOH. The concentrations of the resultant solutions were checked before and after (48 h) exposure using a UV–visible spectrophotometer.
As the experiment starts neonate were placed in 50 ml of beaker with 40 ml of test solution. All experiment was made in four replicates and performed at 21 ± 0.3 degree C under 16 h light: 8 h dark photoperiod.
Immobility was observed after 24 and 48 hrs and EC 50 value was calculated. After the experiment the EC 50 value of test material for Daphnia magna was determined to be 943 mg/l in 48 hrs.
Thus, based on the above summarised studies, 2-(N-ethylamino)-1-(4-methoxyphenil propane) and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range 100.8 to 943.26 mg/L. Thus, comparing this value with the criteria of CLP regulation , 2-(N-ethylamino)-1-(4-methoxyphenil propane) cannot be classified for short term toxicity to aquatic invertebrates .Hence,based on the data available for the structurally and functionally similar read across, test chemical 2-(N-ethylamino)-1-(4-methoxyphenil propane) is not likely to be toxic atleast in the concentration range of 100.8 to 943.26 mg/L
.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 943.26 mg/L
Additional information
Short-term toxicity to aquatic invertebrates:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity to aquatic invertebrates of the test chemical 2-(N-ethylamino)-1-(4-methoxyphenil propane) (14367 -46 -5).The studies are as mentioned below:
1.Determination of the inhibition of the mobility of Daphnids was carried out with the test materialaccording to OECD Guideline 202.
The stock solution 150.0 mg/L was prepared by dissolving white powder in reconstituted water. The solution was kept in ultrasonic bath for 20 min. The test solutions of required concentrations were prpared by mixing the stock solution of the test sample in reconstituted water.The test substance was tested at the concentrations 0, 30.0, 45.0, 67.5, 100.0 and 150.0 mg/L.The test was performed under static conditions in a fresh water system at a temperature of 20 °C± 1 °C. EC50 was calculated using non linear regression by the software Prism 4.0.
The median effective concentration (EC50) for the test substance, test material, in Daphnia magna was determined to be 100.8 mg/L for immobilisation effects with 95% CI of 94.7 to 107.4 mg/L.
2.Short term toxicity to aquatic invertebrates was performed in Daphnia magna for 48 hrs. The young daphnids were obtained from continuous cultures in 1 liter glass beakers at 21 ± 0.3 degree C, in dechlorinated and conditioned tap water, the medium was renewed in three weeks and daphnids were fed daily with the green alga Selenastrum capricornutum. The pH of test solution was adjusted to 7.45 ± 0.05 with HCL and NaOH. The concentrations of the resultant solutions were checked before and after (48 h) exposure using a UV–visible spectrophotometer.
As the experiment starts neonate were placed in 50 ml of beaker with 40 ml of test solution. All experiment was made in four replicates and performed at 21 ± 0.3 degree C under 16 h light: 8 h dark photoperiod.
Immobility was observed after 24 and 48 hrs and EC 50 value was calculated. After the experiment the EC 50 value of test material for Daphnia magna was determined to be 943 mg/l in 48 hrs.
Thus, based on the above summarised studies, 2-(N-ethylamino)-1-(4-methoxyphenil propane) and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range 100.8 to 943.26 mg/L. Thus, comparing this value with the criteria of CLP regulation , 2-(N-ethylamino)-1-(4-methoxyphenil propane) cannot be classified for short term toxicity to aquatic invertebrates .Hence,based on the data available for the structurally and functionally similar read across, test chemical 2-(N-ethylamino)-1-(4-methoxyphenil propane) is not likely to be toxic atleast in the concentration range of 100.8 to 943.26 mg/L
.
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