1,4,6,10-Dodecatetraen-3-ol,3,7,11-trimethyl-,(4E,6E)-

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study; no deviations

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,4,6,10-Dodecatetraen-3-ol,3,7,11-trimethyl-,(4E,6E)-

EC Number:

611-799-6

Cas Number:

59121-99-2

Molecular formula:

C15H24O

IUPAC Name:

1,4,6,10-Dodecatetraen-3-ol,3,7,11-trimethyl-,(4E,6E)-

Test material form:

liquid: viscous

Details on test material:

- Name of test material (as cited in study report):Vinylpseudoionol
- Molecular formula (if other than submission substance): C15H24O
- Molecular weight (if other than submission substance): 220.36 g/mol
- Physical state: viscous-liquid
- Analytical purity: 87.1 %
- Impurities (identity and concentrations): 1.2 % water
- Purity test date: not indicated
- Lot/batch No.: technikumscharge M 401
- Expiration date of the lot/batch: not indicated
- Storage condition of test material: storage at room temperature under nitrogen
- Water solubility: poorly soluble

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic (adaptation not specified)

Details on inoculum:

Activated sludge from laboratory wastewater plants treating municipal sewage . Concentration of dry substance
30 mg/L

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

25 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

20 mg/L

Based on:

other: TOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium:
- Additional substrate: no
- Test temperature: 20 - 25 °C
- pH: 7.0 - 7.4
- pH adjusted: yes
- Aeration of dilution water: yes (CO2 free air)
- Suspended solids concentration: 7.5 ml/L inoculum (6 g/l dry matter)
- Continuous darkness: yes/no
- Other:

TEST SYSTEM
- Culturing apparatus: brown glass bottles
- Number of culture flasks/concentration: 2 per test concentration, 1 per controls
- Test performed in open system: yes
- Details of trap for CO2 and volatile organics if used: CO2 trap according to OECD 301 B


SAMPLING
- Sampling days: 0, 1, 3, 5, 7, 10, 12 14, 17, 21, 24, 27, 29
- Sample storage before analysis: not relevant as the samples were analysed asap after sampling


CONTROL AND BLANK SYSTEM
- Inoculum blank: 1
- Abiotic sterile control: 1
- Toxicity control: 1

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

0 - 10

Sampling time:

3 d

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

10 - 20

Sampling time:

7 d

% Degradation 3

Parameter:

% degradation (CO2 evolution)

Value:

60

Sampling time:

12 d

% Degradation 4

Parameter:

% degradation (CO2 evolution)

Value:

60 - 70

Sampling time:

14 d

% Degradation 5

Parameter:

% degradation (CO2 evolution)

Value:

90 - 100

Sampling time:

27 d

BOD5 / COD results

Results with reference substance:

day 0: 0 %
day 3: -1 %
day 5: 27 %
day 14: 60 - 70 %
day 28: 70 - 80 %