Reaction products of 4-amino-3-hydroxy-7-nitronaphthalene-1-sulfonic acid and 2′,4′-dihydroxyacetophenone chelated with iron, sodium salts

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

Source study has reliability 2, due to an exposure time of only 24 h. Details on the read across are available in section 13.

Principles of method if other than guideline:

Test method according to German standard DIN 38412 part 11.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: test solutions were prepared by a serial dilution of a stock solution (500 mg/l).

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 6-24 h.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Hardness:

2.5 ± 0.3 mmol/l

Test temperature:

20 ± 1°C

pH:

7.7 - 8.3

Dissolved oxygen:

8.24 - 8.67 mg/l

Nominal and measured concentrations:

Nominal test substance concentrations:
0, 62.5, 125, 250 and 500 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: reagent tubes with flat bottom
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Ca/Mg ratio: 4:1
- Conductivity: 550 - 650 µS/cm

OTHER TEST CONDITIONS
- Photoperiod: permanent darkness

EFFECT PARAMETERS MEASURED
- Mobility after 0 and 24 hours

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Conclusions:

EC50 (24h) > 500 mg/l

Executive summary:

Method:

Acute toxicity to aquatic invertebrates was tested following German standard DIN 38412 part 11, using nominal test substance concentrations of 0, 62.5, 125, 250 and 500 mg/l. Per each concentration, 20 daphnids were tested, divided in 4 replicates. Effects in terms of mobility were recorded 0 and 24 h after start of exposure.

Results:

EC50 (24h) > 500 mg/l.

Description of key information

Daphnia magna, EC₅₀ (24h) > 500 mg/l (nominal)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

500 mg/L

Additional information

No data on target substance was available, thus a read across approach was followed, using data on Similar Substance 01. Details are reported in section 13.

Acute toxicity to aquatic invertebrates (Daphnia magna) was assessed according to German standard DIN 38412 part 11. Nominal concentrations on Similar Substance 01 were 0, 62.5, 125, 250 and 500 mg/l, divided in 4 replicates with 5 daphnids each, per concentration. Effects in terms of mobility were recorded 0 and 24 h after start of exposure.