Reaction products of 4-amino-3-hydroxy-7-nitronaphthalene-1-sulfonic acid and 2′,4′-dihydroxyacetophenone chelated with iron, sodium salts

Endpoint summary

Description of key information

Short-term toxicity to fish: LC50 (96h) = 20.7 mg/l

Short-term toxicity to aquatic invertebrates: EC50 (24h) > 500 mg/l

Toxicity to aquatic algae: EC50 (72h) = 71.8 mg/l

Additional information

The assessement on aquatic toxicity of target substance is based on a read across approach using available data on Similar Substance 01. Both target substance and Similar Substance 01 are soluble in water and have a negative partition coefficient.

Further details on the read across are reported in section 13.

Available data on fish, aquatic invertebrates and algae were taken into account.

Short-term toxicity to fish

Mortality was seen in a 96 h study under static exposure conditions, using test concentrations up to 100 mg/l. LC50 (96h) = 20.7 mg/l.

Short-term toxicity to aquatic invertebrates

Effects on mobility were recorded up to 24 h after start of exposure. EC50 (24h) > 500 mg/l.

Toxicity to aquatic algae

Effects on growth rate and biomass were seen in a 72 h study under static exposure conditions, using concentrations up to 100 mg/l. EC50 (72h) = 71.8 mg/l.

Justification for classification or non-classification

According to the CLP Regulation (EC 1272/2008), the threshold of classification of a substance for acute aquatic toxicity is 1 mg/l. This limit is compared with LC50 (96h ) for fish, EC50 (48h) for daphnia, EC50 (72 -96 h) for algae and EC50 (7 d) for other aquatic plants.

As for chronic toxicity, in case of lack of chronic data, the toxic potential is identified by appropriate combinations of acute toxicity data and lack of biodegradability. The threshold of classification is 100 mg/l.

Based on findings in fish and algae, a classification in category 3 for chronic aquatic toxicity (H412) was applied within the CLP Regulation (EC 12727/2008).