Butyl carbamate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From March 21, 2016 to April 15, 2016

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

No analytical verification of test concentrations

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes

Specific details on test material used for the study:

Batch no.: IIUB 005
Purity: ca. 94%
Appearance: colorless, crystallized melt
Solubility in water: <0.1%

Analytical monitoring:

no

Vehicle:

yes

Remarks:

water

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Source: Hoechst AG (Eigenzucht der Gewerbetoxikologie)
Size: 2.5-3.1 cm
Temperature: 22 ± 1°C
Oxygen content: ≥80 %
Light period: 12h
Feeding: Tetra Min, Tetra Werke, Melle (ad libitum)
Fish concentration: ≤1 g fish/L water

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

-

Test temperature:

21.4-2.5

pH:

7.6-8.2

Dissolved oxygen:

3.4-8.9 mg/L

Salinity:

-

Conductivity:

-

Nominal and measured concentrations:

250, 350, and 500 mg/L

Details on test conditions:

10 (fish/test conditions)

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

350 - 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 329-470 mg/L (a.i.)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 186 mg/L (a.i.)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 470 mg/L (a.i.)

Details on results:

Test conditions:
- 250 mg/L: mortality of 0
- 350 mg/L: mortality of 2/10 after 24h
- 500 mg/L: mortality of 10/10 after 2-4h

Validity criteria fulfilled:

not specified

Conclusions:

Under the study conditions, the nominal 96 h LC50 of the test substance for zebra fish (Brachydanio rerio) was determined to be 350 - 500 mg/L (equivalent to 329-470 mg a.i./L based on a purity of 94%).

Executive summary:

A study was conducted to determine the short-term toxicity of the test substance to zebra fish (Brachydanio rerio) under static conditions according to OECD Guideline 203 and EU Method C.1. The fish (10/group) were exposed for 96 h to nominal concentrations of 0, 250, 350 and 500 mg/L (equivalent to 0, 235, 329 and 470 mg a.i./L based on a purity of 94%). Mortality and symptoms were assessed after 2 -4, 24, 48, 72 and 96 h. No analytical verification of doses was conducted. There was no mortality at 250 mg/L. Two out of 10 fish died after 24 h in the 350 mg/L group. Finally, a mortality of 100% was observed after 2 -4 h when fish were exposed to 500 mg/L test substance. Under the study conditions, the 96 h LC50 of the test substance was determined to be 350 - 500 mg/L, equivalent to 329-470 mg a.i./L (Markert and Jung, 1988).

Description of key information

The 96 h EC50 of the substance was considered to be 350 mg/L (i.e., equivalent to 329 mg a.i./L).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

329 mg/L

Additional information

A study was conducted to determine the short-term toxicity of the test substance to zebra fish (Brachydanio rerio) under static conditions according to OECD Guideline 203 and EU Method C.1. The fish (10/group) were exposed for 96 h to nominal concentrations of 0, 250, 350 and 500 mg/L (equivalent to 0, 235, 329 and 470 mg a.i./L based on a purity of 94%). Mortality and symptoms were assessed after 2 -4, 24, 48, 72 and 96 h. No analytical verification of doses was conducted. There was no mortality at 250 mg/L. Two out of 10 fish died after 24 h in the 350 mg/L group. Finally, a mortality of 100% was observed after 2 -4 h when fish were exposed to 500 mg/L test substance. Under the study conditions, the 96 h LC50 of the test substance was determined to be 350 - 500 mg/L, equivalent to 329-470 mg a.i./L (Markert and Jung, 1988).