4-methoxyphenylacetone

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

No data

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from peer reviewed publication

Data source

Reference

Reference Type:

publication

Title:

Study of artificial flavouring substances for mutagenicity in the Salmonella/Microsome Basc and Micronucleus tests

Author:

D. Wild, M. T. King, E. Gocke and K. Eckhardt

Year:

1983

Bibliographic source:

Food and chemical toxicology, Vol. 21, 6, 707-719, 1983

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Principles of method if other than guideline:

The mutagenic potential of 4-(4-Methoxyphenyl)butan-2-one (Anisyl acetone) was determned by conducting experiment using Salmonella typhimurium TA1535, TA100, TA1537, TA1538 and TA 98.

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

4-(4-methoxyphenyl)butan-2-one

EC Number:

203-184-2

EC Name:

4-(4-methoxyphenyl)butan-2-one

Cas Number:

104-20-1

Molecular formula:

C11H14O2

IUPAC Name:

4-(4-methoxyphenyl)butan-2-one

Details on test material:

- Name of test material: 4-(4-Methoxyphenyl)butan-2-one (Anisyl acetone)
- IUPAC name: 4-(4-methoxyphenyl)butan-2-one
- Molecular formula: C11H14O2
- Molecular weight: 178.23 g/mol
- Substance type: Organic

Specific details on test material used for the study:

- Name of test material: 4-(4-Methoxyphenyl)butan-2-one (Anisyl acetone)
- IUPAC name: 4-(4-methoxyphenyl)butan-2-one
- Molecular formula: C11H14O2
- Molecular weight: 178.23 g/mol
- Substance type: Organic
- Physical state: No data
- Purity: No data
- Impurities (identity and concentrations): No data

Method

Target gene:

Histidine

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA1535, TA100, TA1537, TA1538, TA98

Details on mammalian cell type (if applicable):

Not applicable

Additional strain / cell type characteristics:

not specified

Cytokinesis block (if used):

not specified

Metabolic activation:

with and without

Metabolic activation system:

S9 mixture from liver fraction of rat pretreated with Aroclor 1254

Test concentrations with justification for top dose:

Up to 3.6 mg/plate

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: Solubility of test substance in DMSO solvent.

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

sodium azide

benzo(a)pyrene

Details on test system and experimental conditions:

METHOD OF APPLICATION: In agar (plate incorporation)

DURATION
- Preincubation period: No data
- Exposure duration: 48 hrs
- Expression time (cells in growth medium): No data
- Selection time (if incubation with a selection agent): No data
- Fixation time (start of exposure up to fixation or harvest of cells): No data

SELECTION AGENT (mutation assays): No data
SPINDLE INHIBITOR (cytogenetic assays): No data
STAIN (for cytogenetic assays): No data

NUMBER OF REPLICATIONS: Two

NUMBER OF CELLS EVALUATED: No data

DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data

OTHER EXAMINATIONS:
- Determination of polyploidy: No data
- Determination of endoreplication: No data

Rationale for test conditions:

No data

Evaluation criteria:

The plates were observed for a dose dependent increase in number of revertants.

Statistics:

Statistical significance was determined according to the methods of Kastenbaum & Bowman

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA1535, TA100, TA1537, TA1538, TA98

Metabolic activation:

with and without

Genotoxicity:

not specified

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

not valid

Positive controls validity:

valid

Additional information on results:

No data

Remarks on result:

other: No mutagenic potential

Applicant's summary and conclusion

Conclusions:

4-(4-Methoxyphenyl)butan-2-one (Anisyl acetone) did not induce gene mutation in Salmonella typhimurium strains TA1535, TA100, TA1537, TA1538, TA98, test substance in the presence and absence of S9 metabolic activation system and hence it is not likely to classify as a gene mutant.

Executive summary:

Gene mutation toxicity study was performed to determine the mutagenic nature of 4-(4-Methoxyphenyl)butan-2-one (Anisyl acetone). The study was perfomed as per the standard plate procesdure using Salmonella typhimurium strains TA1535, TA100, TA1537, TA1538, TA98 in the presence and absence of S9 metabolic activation system. The test chemical was dissolved in DMSO and used upto doses of 3.6 mg/plate. The plates were incubated for 48 hrs. 4-(4-Methoxyphenyl)butan-2-one (Anisyl acetone) did not induce gene mutation in Salmonella typhimurium strains TA1535, TA100, TA1537, TA1538, TA98, test substance in the presence and absence of S9 metabolic activation system and hence it is not likely to classify as a gene mutant.