2,2'-[ethylenebis(oxy)]bisacetic acid

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

October - November 1990

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

Adopted: 04. April 1984

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

guideline of commission 25. April 1984

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Sampling method: With a bottle 100 mL were sampled in the middle of the tank

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
The test substance was diluted with dilutionwater, homogenised with a ultrasonic bath and Ultra-Turrax.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Strain: HAMILTON-BUCHANAN
- Source: West Aquarium, 3422 Bad Lauterberg
- Length at study initiation: range: 2.7 - 3.3 cm; mean: 3.1 cm
- Weight at study initiation: not available

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: same as test conditions
- Type and amount of food during acclimation: Tetra Min, Tetra Werke, Melle
- Feeding frequency during acclimation: twice a day
- Health during acclimation: no mortality observed

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

21.3 - 21.5 °C

pH:

7.7 - 8.3

Dissolved oxygen:

7.4 - 9.4 mg/L

Nominal and measured concentrations:

Nominal concentration: 500 mg/L
Measured concentration: 497 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass tank in water bath
- Material, fill volume, size: glass, 10L, 30cm x 22cm x 24cm
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: < 1 g fish/L water

TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: Following ISO/DIS 7346/1

OTHER TEST CONDITIONS
- Adjustment of pH: yes, with 10 M NaOH
- Photoperiod: 12 h light, 12 h dark
- Light intensity: ca 700 Lux

EFFECT PARAMETERS MEASURED
Mortality, ability to swim, breathing and water quality paramers were recorded after 2-4, 24, 48, 72 and 96 hours.

Pretest: yes, but no details availabe

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: no
- Observations on body length and weight: no abnormalities
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no

Sublethal observations / clinical signs:

Sampling date	1990-11-05		1990-11-07		1990 -11-09		Geometric mean measured concentration [mg/L]
Nominal concentration	Meas. conc. [mg/L]	%	Meas. conc. [mg/L]	%	Meas. conc. [mg/L]	%
Control	<1.0		<1.0		<1.0
500 mg/L	500	100	494	98.8	497	99.4	497

Meas. conc. = Measured concentration of the test item, enrichment and dilution factor taken into account

% = Percent of nominal concentration of test item

Geometric mean measured test item concentrations [mg/L]	Observation*	Number of fish effected after different exposure periods [hours]
		3	24	48	72	96
500	N	7	7	7	7	7
Control	N	7	7	7	7	7

*The letter correspond to the following observations:

N = Normal behavior

Validity criteria fulfilled:

yes

Conclusions:

In this study the test item was found to cause no lethal effects to zebrafish after 96 hours at a concentration of 500 mg test item/L. The LC50 after 96 hours was > 500 mg test item/L.

Executive summary:

The acute toxicity of the test item to fish (zebrafish) was determined according to the principles of the OECD-Guideline for Testing of Chemicals No. 203 (1984) and EG Guideline C.1 (1984) in 1990. An acute toxicity test under static conditions was conducted with the nominal test item concentration of 500.0 mg/L. Duration of the test was 96 hours. Ten test organisms were exposed to the test concentration and the control. Water quality parameters (temperature, pH-value and oxygen- saturation) measured at 2 -4, 24, 48, 72 and 96 hours were determined to be within the acceptable limits. The determination of the test concentrations was carried out via HPLC (UV). Evaluation was carried out from the media after 0, 48 and 96 h. The measured concentration was 497 mg/L, less than 20% below the nominal concentration. Therefore, all effect values are based on nominal concentrations. All validity criteria of the test guideline were met. The LC50 was determined to be > 500 mg/L.

Description of key information

The test item was found to cause no lethal effects to zebrafish after 96 hours at a concentration of 500 mg test item/L. The LC50 after 96 hours was > 500 mg test item/L.

Additional information

The acute toxicity of the test item to fish (zebrafish) was determined according to the principles of the OECD-Guideline for Testing of Chemicals No. 203 (1984) and EG Guideline C.1 (1984) in 1990. An acute toxicity test under static conditions was conducted with the nominal test item concentration of 500.0 mg/L. Duration of the test was 96 hours. Ten test organisms were exposed to the test concentration and the control. Water quality parameters (temperature, pH-value and oxygen- saturation) measured at 2 -4, 24, 48, 72 and 96 hours were determined to be within the acceptable limits. The determination of the test concentrations was carried out via HPLC (UV). Evaluation was carried out from the media after 0, 48 and 96 h. The measured concentration was 497 mg/L, less than 20% below the nominal concentration. Therefore, all effect values are based on nominal concentrations. All validity criteria of the test guideline were met. The LC50 was determined to be > 500 mg/L.