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EC number: 222-824-1 | CAS number: 3623-51-6
Immobilisation of Daphnia magna after an exposure of 24 h
Immobilisation of Daphnia magna
(initial population: 20)
Immobilisation of Daphnia magna after an exposure of 48 h
Immobilisation ofDaphnia magna
The acute toxicity of the test item, was tested in an immobilisation assay in Daphnia magna. The assay was conducted according to EU method C.2. The test organisms were exposed the substance at nominal concentrations of 3.2, 6.3, 12.5, 25, 50, and 100 mg/L for up to 48 h. Test subjects were observed for alterations of the normal mobility behaviour and loss of locomotory activity. From the data obtained through these observations, the effective concentration 0% (EC0) and the effective concentration 100% (EC100) for immobilisation induced by an exposure to the test item for 24 h were determined as 25 and 100 mg/L, respectively. For an exposure period of 48 h, the respective values were 11.35 and 92.35 mg/L. Furthermore, effective concentration 50% (EC50) values of 37.7 and 26.6 mg/L were determined for the 24 h and the 48 h exposure, respectively.
Daphnia magna EC50 (48 h) = 26.6 mg/L (measured); read-across
Since no data for the target substance are available, the aquatic toxicity is assessed on the basis of data from one structurally similar menthol substance (stereo isomer). The acute toxicity of L-menthol (CAS 2216-51-5) was tested in a static immobilisation test with the water flea Daphnia magna as test organism. The study was conducted according to EU method C.2 (GLP). The test organisms were exposed to nominal concentrations of 3.2, 6.3, 12.5, 25, 50, and 100 mg/L for up to 48 h. Test organisms were observed for alterations of the normal mobility behaviour and loss of locomotory activity. From the data obtained during these observations, the effective concentration 0% (EC0) and the effective concentration 100% (EC100) for immobilisation induced by an exposure to the test item for 48 h were determined as 11.35 and 92.35 mg/L, respectively. The effective concentration 50% (EC50) value was calculated to be 26.6 mg/L.
Based on the result from one structurally related source substance (in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5) it can be concluded that testing the target substance would result in comparable results.
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