Tin antimony grey cassiterite

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

2000-03-13 to 2000-03-17

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

yes

Principles of method if other than guideline:

- actual concentrations were not measured.
- only a brief description of the test conditions is given

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Tin antimony grey cassiterite

EC Number:

269-105-9

EC Name:

Tin antimony grey cassiterite

Cas Number:

68187-54-2

Molecular formula:

Sn(2-x-y)Sb(x)V(y)O4 0,04≤x≤0,50 0≤y≤0,10

IUPAC Name:

Tin antimony cassiterite

Test material form:

solid: particulate/powder

Details on test material:

- Name of test material: Tin-Antimony-Grey
- Substance type: inorganic pigment
- Physical state: solid, grey powder
- Stability under test conditions: at least 96 hours
- Storage condition of test material: At room temperature in the dark

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
not applicable

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

no data

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method:
The test solution was prepared at 100 mg/L applying magnetic stirring for 45 minutes followed by 1 minute of treatment with ultrasonic waves. The final test solution was a grey suspension with some undissolved particles.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra-fish (Danio rerio, Teleostei, Cyprinidae)
- Source: Zodiac, proefacc. "De Haar Vissen", L.U. Wageningen, the Netherlands
- Length at study initiation (mean ± SD): 2.29 ± 0.11 cm
- Weight at study initiation (mean ± SD): 0.25 ± 0.04 g
- Characteristics: F1 from a single parent-pair bred in UV-treated water

Study design

Test type:

static

Water media type:

not specified

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

218 mg CaCo3/L

Test temperature:

20.9-22.2 °C

pH:

- Control (blank):
test start: 7.9
test end: 7.8
- Test vessel:
test start: 7.8
test end: 7.9

Dissolved oxygen:

- Control (blank):
test start: 10.0 mg O2/L
test end: 8.4 mg O2/L
- Test vessel:
test start: 10.1 mg O2/L
test end: 8.9 mg O2/L

Salinity:

not applicable

Nominal and measured concentrations:

Nominal concentration: 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 6 litres, all glass
- Aeration: solutions were gently aerated as oxygen concentrations tend to decrease below 6 mg/L.
- No. of organisms per vessel: 7
- Loading: 0.35 g fish per litre (i.e. 7 fish per 5 litres of test medium)

TEST MEDIUM / WATER PARAMETERS
- test water: ISO-medium (hardness: 218 mg CaCO3/L)

EFFECT PARAMETERS MEASURED
- incidence of mortality and total mortality

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Deposits were observed during the entire test period at 100 mg/L.

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Deposits were observed during the entire test period at 100 mg/L.

Details on results:

Tin-antimony-grey did not affect zebra-fish at 100 mg/L during the 96-hour test.

Any other information on results incl. tables

Sublethal observations / clinical signs:

Table 1: Incidence of mortality and total mortality during the limit test

TIN-ANTIMONY-GREY	Initial number of fish	Cumulative mortality					Total mortality (%)
		3.25 hours	24 hours	48 hours	72 hours	96 hours
Blank-control	7	0	0	0	0	0	0
100*	7	0	0	0	0	0	0

* Deposits were observed during the entire test period in the 100 mg/L solution.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

TIN-ANTIMONY-GREY did not affect zebra-fish at 100 mg/L (nominal, dispersed) during the 96-hour limit test according to OECD TG 203, and the 96-h LC50 is estimated with > 100 mg/L (nominal diospersed) and the corresponding NOEC with >= 100 mg/L (nominal, dispersed).