2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Title:

Unnamed

Year:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride

EC Number:

303-085-5

EC Name:

2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride

Cas Number:

94158-14-2

Molecular formula:

C9H11NO3.ClH

IUPAC Name:

2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride

Test material form:

solid: crystalline

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Test temperature:

18-22 °C +/- 1 °C

Results and discussion

Effect concentrations

Key result

Duration:

24 h

Dose descriptor:

EC10

Effect conc.:

2.65 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

The Acute Immobilisation (EC50) of the test item AMINOL (batch number: R0075037), to Daphnia magna (STRAUS) was determined according to OECD Guideline 202 (1999, Draft)/EC Directive 92/69/EC Method C.2 from 2003-08-26 to 2003-08-28 at the DR.U.NOACK-LABORATORIUM FÜR ANGEWANDTE BIOLOGIE, D-31157 Sarstedt. The study was conducted under static conditions over a period of 48 hours. 20 test organisms were exposed to each test concentration and control. The recovery rates of the test item in the new and the old media were > 80 %, therefore the results are given based on the nominal test item concentrations. The nominal test item concentrations were as follows: 10.0 - 5.00 - 2.50 - 1.25 - 0.625 mg/L. The 24 and 48 h EC10-values and the 24 and 48 h EC50-values with confidence interval (Cl) were calculated by probit analysis (Table 1 ). The 24 and 48 h EC100-values were determined directly from the test results obtained. A reference test was carried out with potassium dichromate. The EC50-value of the reference item at 1.49 mg/L after 24 h was within the prescribed concentration range of 1.0 to 2.5 mg/L according to AQS, DIN Guideline 38412 L 30. Water quality parameters pH-value and dissolved oxygen concentration measured at 0 and 48 h were determined to be within the acceptable Iimits. The validity criteria of the test guideline were fulfilled.

Table 1: EC10-, EC50- (with Confidence lnterval), EC100- Values

(based on nominal test item concentrations)

	Test duration [h]	Concentration [mg/L]	Confidence interval (p=95%) [mg/L]
EC10	24	2.65	---
EC10	48	2.15	---
EC50	24	6.01	4.79-7.56
EC50	48	2.67	2.48-2.87
EC100	24	>10.0	---
EC100	48	5.00	---