(2S)-1,3,4,5,6,7-hexahydro-1,1,5,5-tetramethyl-2H-2,4a-methanonaphthalene

Endpoint summary

Description of key information

Short term toxicity to fish:

Based on the prediction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to fish was predicted.

On the basis of this program, the LC 50 value for short term toxicity to fish was predicted to be 0.068 mg/l for test materialin 96 hrs.

Based on this value it can be concluded that the substance is considered to be toxic to aquatic environment and can be classified in aquatic acute 1 category as per the criteria mentioned in CLP regulation.

Toxicity to aquatic invertebrate:

Based on the prediction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted.

On the basis of this program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted to be0.055mg/l for test materialin 48 hrs.

Based on this value it can be concluded that the substance is considered to be toxic to aquatic environment and can be classified in aquatic acute 1 category as per the criteria mentioned in CLP regulation.

Toxicity to aquatic algae and cyanobacteria:

The toxicity of the test substance to green algae is predicted using EPI Suite ECOSAR version 1.11. On the basis of effects observed in a static freshwater system during a 96 hr exposure, the effect concentration (EC50) for the substance is estimated to be0.172mg/L. Based on this value, it can be concluded that the test chemical can be considered as toxic to green algae at environmentally relevant concentrations and can be considered to be classified in aquatic acute 1 as per the CLP classification criteria.

Toxicity to microorganism:

Data available for the test chemicals has been reviewed to determine its effect on microoranism.The studies are as mentioned below:

Toxicity of test material was evaluated for microorganisms by disc diffusion method and the minimum inhibition concentration was evaluated using broth dilution method.The organisms used for the test wereS. aureus, B. cereus , E. coli , P. aeruginosa.The turbidity of microorganisms was adjusted to 0.5 McFarland. The inoculum were 1×10⁸1×10⁶CFU/ml for bacteria and fungi respectively. Inoculate was swabbed on Muller Hinton Agar. Sterile

blank discs impregnated with 3, 5, 10 μl of oil in 10 μl of dimethyl sulfoxide (DMSO) were used and put on cultured plates. Disc containing DMSO and antibiotics were used as control.The minimum inhibition concentration of test material on bacteria S. aureus, B. cereus , E. coli , P. aeruginosa was observed to be in the range of 1 - 8 mg/l.Based on the above effect concentartion it can be considered that test substance is moderately toxic to microorganisms.

 

 

Additional information

Short term toxicity to fish:

The toxicity of test material on fish was predicted and evaluated by various data from data sources:

Based on the prediction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to fish was predicted.

On the basis of this program, the LC 50 value for short term toxicity to fish was predicted to be 0.068 mg/l for test materialin 96 hrs.

Based on this value it can be concluded that the substance is considered to be toxic to aquatic environment and can be classified in aquatic acute 1 category as per the criteria mentioned in CLP regulation.

1)Short term toxicity of fish was evaluated for test material on Pimephales promelas . The median lethal effect concentration (LC50) for fish after 96 h was observed to be 0.28 mg/l . Based on the above effect concentration it can be concluded that the test material is toxic for fish and can be classified as aquatic aute 1 category as per CLP criteria.

2)Short term toxicity of fish was evaluated for test material . The median lethal effect concentration (LC50) for fish after 96 h was observed to be >0.42 mg/l . Based on the above effect concentration it can be concluded that the test material is toxic for fish and can be classified as aquatic aute 1 category as per CLP criteria.

Toxicity to aquatic invertebrate:

Based on the prediction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted.

On the basis of this program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted to be0.055mg/l for test materialin 48 hrs.

Based on this value it can be concluded that the substance is considered to be toxic to aquatic environment and can be classified in aquatic acute 1 category as per the criteria mentioned in CLP regulation.

The above predicition was supported by the data from authorative databases

1)To study the effects of test material on aquatic invertebrate test was carried for 48 H under static condition. Increasing trend of Intoxication (immobile) was measured during the test.Effective concentration EC50 to 50% of Daphnia pulex when exposed to test material for 48 h is 0.044 mg/L. It can be concluded from the EC50 Intoxication value that the test material is toxic to the aquatic invertebrateand can be considered as “Aquatic acute 1” as per the classification criteria for aquatic environment.

2)To study the effects of [1S-(1α,3aβ,4α,8aβ)]-decahydro-4,8,8-trimethyl-9-methylene-1,4-methanoazulene on aquatic invertebrate test was carried for 48 H under static condition. Increasing trend of Intoxication (immobile) was measured during the test.

Effective concentration EC50 to 50% of Daphnia pulex when exposed to 1S-(1α,3aβ,4α,8aβ)]-decahydro-4,8,8-trimethyl-9-methylene-1,4-methanoazulene for 48 h is 0.08 mg/L. It can be concluded from the EC50 Intoxication value that the 1S-(1α,3aβ,4α,8aβ)]-decahydro-4,8,8-trimethyl-9-methylene-1,4-methanoazulene is toxic to the aquatic invertebrateand can be considered as “Aquatic acute 1” as per the classification criteria for aquatic environment.

Toxicity to aquatic algae and cyanobacteria:

Toxicity of test material on aquatic algae and cyanobacteria was evaluated using predicted and experimental data from authorative database:

The toxicity of the test substance to green algae is predicted using EPI Suite ECOSAR version 1.11. On the basis of effects observed in a static freshwater system during a 96 hr exposure, the effect concentration (EC50) for the substance is estimated to be0.172mg/L. Based on this value, it can be concluded that the test chemical can be considered as toxic to green algae at environmentally relevant concentrations and can be considered to be classified in aquatic acute 1 as per the CLP classification criteria.

 

1) Short term toxicity study for the test material was conducted on aquatic algae and cyanobacteria as per OECD Guideline 201 (Alga, Growth Inhibition Test). The EC50 effect concentartion for the test substance on aquatic algae was obswerved to be >0.16 mg/l , the NOEC was observed to be 0.16 mg/l. Based on the above effect concentration it can be concluded that test material is toxic to aquatic algae and can be classified as aquatic acute 1 as per CLP criteria.

2)Alga, Growth Inhibition Test was carried with Pseudokirchneriella subcapitata, 72 hours for test material according to OECD Guideline 201 (Alga, Growth Inhibition Test).

The study was conducted under static conditions with an initial cell density of 5089 cells/mL. With regard to the volatility of the test item glass flasks without headspace were used to reduce losses of test item. Seven concentrations were tested in a geometrical series with a dilution

factor of 3.16 (nominal): 0.00500 - 0.0158 - 0.0500 - 0.158 - 0.500 - 1.58 - 5.00 mg/L, corresponding to geometric mean measured test item concentrations of 0.00467 - 0.0153 -0.0445 - 0.153 - 0.405 - 1.36 - 4.39 mgIL. Effective concentration EC50 to 50% of Pseudokirchneriella subcapitata when exposed to test material, for 72h is 0.72 mg/L.

It can be concluded from the EC50 Intoxication value that the test material is toxic to the aquatic algae and can be considered as “Aquatic acute 1” as per the classification criteria for aquatic environment.

Toxicity to microorganism:

Data available for the test chemicals has been reviewed to determine its effect on microoranism.The studies are as mentioned below:

Toxicity of test material was evaluated for microorganisms by disc diffusion method and the minimum inhibition concentration was evaluated using broth dilution method.The organisms used for the test wereS. aureus, B. cereus , E. coli , P. aeruginosa.The turbidity of microorganisms was adjusted to 0.5 McFarland. The inoculum were 1×10⁸1×10⁶CFU/ml for bacteria and fungi respectively. Inoculate was swabbed on Muller Hinton Agar. Sterile

blank discs impregnated with 3, 5, 10 μl of oil in 10 μl of dimethyl sulfoxide (DMSO) were used and put on cultured plates. Disc containing DMSO and antibiotics were used as control.The minimum inhibition concentration of test material on bacteria S. aureus, B. cereus , E. coli , P. aeruginosa was observed to be in the range of 1 - 8 mg/l.Based on the above effect concentartion it can be considered that test substance is moderately toxic to microorganisms.

1)Toxicity of test material was evaluated for microorganisms by disc diffusion method and the minimum inhibition concentration was evaluated using broth dilution method.The organisms used for the test wereS. aureus, B. cereus , E. coli , P. aeruginosa.The turbidity of microorganisms was adjusted to 0.5 McFarland. The inoculum were 1×10⁸1×10⁶CFU/ml for bacteria and fungi respectively. Inoculate was swabbed on Muller Hinton Agar. Sterile blank discs impregnated with 3, 5, 10 μl of oil in 10 μl of dimethyl sulfoxide (DMSO) were used and put on cultured plates. Disc containing DMSO and antibiotics were used as control.The minimum inhibition concentration of test material on bacteria S. aureus, B. cereus , E. coli , P. aeruginosa was observed to be >8 mg/l.

2) Toxicity of test material was evaluated for microorganisms by disc diffusion method and the minimum inhibition concentration was evaluated using broth dilution method.The organisms used for the test wereS. aureus, B. cereus , E. coli , P. aeruginosa.The turbidity of microorganisms was adjusted to 0.5 McFarland. The inoculum were 1×10⁸1×10⁶CFU/ml for bacteria and fungi respectively. Inoculate was swabbed on Muller Hinton Agar. Sterile

blank discs impregnated with 3, 5, 10 μl of oil in 10 μl of dimethyl sulfoxide (DMSO) were used and put on cultured plates. Disc containing DMSO and antibiotics were used as control.The minimum inhibition concentration of test material on bacteria S. aureus, B. cereus , E. coli , P. aeruginosa was observed to be 1 mg/l