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EC number: 274-349-4 | CAS number: 70161-14-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
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- Nanomaterial pour density
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989-10-24 to 1990-04-02
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Version / remarks:
- adopted 24 February 1987
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Trisodium 7-[[2-[(aminocarbonyl)amino]-4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]phenyl]azo]naphthalene-1,3,5-trisulphonate
- EC Number:
- 274-349-4
- EC Name:
- Trisodium 7-[[2-[(aminocarbonyl)amino]-4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]phenyl]azo]naphthalene-1,3,5-trisulphonate
- Cas Number:
- 70161-14-7
- Molecular formula:
- C20H16ClN9O10S3.3Na
- IUPAC Name:
- trisodium 7-({4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-2-(carbamoylamino)phenyl}diazenyl)naphthalene-1,3,5-trisulfonate
- Test material form:
- solid: particulate/powder
- Details on test material:
- Reactive Orange 12
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Borchen, Germany
- Females (if applicable) nulliparous and non-pregnant: yes
- Age at study initiation: males: 8 weeks (mean), females: 10 weeks (mean)
- Weight at study initiation: Males: 180 g (mean), females 172 g (mean)
- Fasting period before study: 16 hours before and 4 hours after treatment
- Housing: Animals were kept in Makrolon cages type III on dust-free wood granules
- Diet (e.g. ad libitum): Altromin R 1324 from Altromin GmbH, Lage, Germany; ad libitum
- Water (e.g. ad libitum): tap water; ad libitum
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 2
- Humidity (%): 50 ± 10
- Photoperiod (hrs dark / hrs light): 12 / 12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- not specified
- Doses:
- 2000 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: weighing: immediately before application, weekly
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs - Statistics:
- n.a.
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000
- Based on:
- test mat.
- Remarks on result:
- not determinable due to absence of adverse toxic effects
- Mortality:
- No deaths
- Clinical signs:
- No effects observed
- Body weight:
- No effects observed. For individual results please refer to Table 1 in box "Any other information on results incl. tables"
- Gross pathology:
- No effects, except one finding in one male animal (elastic knot in the glandular gastric mucosa).
Any other information on results incl. tables
Table 1: Individual body weights
Males |
Females | ||||||
Animal No. | Week | Animal No. | Week | ||||
0 | 1 | 2 | 0 | 1 | 2 | ||
1 | 170 | 212 | 237 | 6 | 167 | 178 | 184 |
2 | 188 | 231 | 266 | 7 | 175 | 186 | 199 |
3 | 176 | 207 | 227 | 8 | 176 | 193 | 201 |
4 | 188 | 231 | 260 | 9 | 170 | 179 | 203 |
5 | 178 | 221 | 242 | 10 | 172 | 192 | 196 |
Mean | 180 | 220 | 246 | 172 | 186 | 197 |
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- In an acute oral toxicity study, the LD50 of the test item was determined to be greater than 2000 mg/kg bw in rats.
- Executive summary:
In an acute oral toxicity study, young adult Wistar rats (5/sex) were given a single oral dose of the test item at a dose of 2000 mg/kg bw and were observed for 14 days. All animals survived until the end of the study. No effects on body weight and no adverse signs of toxicity were observed. Gross pathology performed on animals revealed no adverse signs, except one finding in one male animal, which showed a elastic knot in the glandular gastric mucosa. Based on the results, the oral LD50 in both male and female rats can be considered to be greater than 2000 mg/kg bw. Based on these results, no classification for acute oral toxicity is warranted according to CLP criteria.
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