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EC number: 226-033-2 | CAS number: 5235-82-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February 24, 1998 to May 13, 1998
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: While the study was conducted according to OECD TG 202 and in accordance with the Principles of Good Laboratory practice (GLP), the stability of the test substance under test conditions was not verified analytically.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- Certain minor deviations (eg. non verfification of the instrument used to measure conductivity and others) were noted, overall these did not impact the outcome of the study
- Principles of method if other than guideline:
- not applicable
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
not applicable - Analytical monitoring:
- no
- Details on sampling:
- not applicable
- Vehicle:
- yes
- Details on test solutions:
- Dimethylformamide was used as a vehicle to dissolve the test material before adding to the dilution water for both the range finding and definitive study.
For the difinitive test, a stock solution with a nominal concentration of 100000 mg/L was prepared by bringing 5 grams of the test substance to a total volume 50 ml with dimethylformamide. Appropriate volumes of the stock solution were added to test vessels containing 15 liters of dilution water. Nominal concentrations used were 1.3, 2.2, 3.6, 6.0 and 10 mg/L. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain: Daphnia magna
- Source: in-house from a culture originally procured from Aquatic BioSystems Inc., USA
- Age at study initiation: Juvenile daphnids < 24 hours old were used
- Method of breeding: Prior to testing, the daphnid culture was maintained in 100% dilution water under static, renewal conditions and the test organisms were not treated for disease. During the 48 hours prior to begining of the test there was no mortality and at the begining of the test, organisms were apparently free of disease, injuries and abnormalities.
- Feeding during test: not fed during the test
- Food type: The daphnids were fed with freshwater algae, Selenastrum capricornutum, and a mixture of yeast and trout chow (Lot number DC02) once each day before the test.
ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): yes
- The daphnids were fed with freshwater algae, Selenastrum capricornutum, and a mixture of yeast and trout chow (Lot number DC02) once each day before the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- not applicable
- Hardness:
- 160 mg/l as CaCO3
- Test temperature:
- ranged between 19.5-20.8 °C
- pH:
- ranged between 7.7-7.9
- Dissolved oxygen:
- ranged between 8.6 - 8.8 mg/l
- Salinity:
- not applicable
- Conductivity:
- 570 - 580 umhos/cm
- Nominal and measured concentrations:
- Range finding test - 0.10, 1.0 and 10 mg/L (nominal) + solvent control + dilution water control
Definitive study - 1.3, 2.2, 3.6, 6.0 and 10 mg/L (nominal) + solvent control + dilution water control - Details on test conditions:
- A range finding test with 4-(3-(1-naphthylamino)propyl) morpholine was conducted with three concentrations (0.10, 1.0 and 10 mg/L), a solvent control (86 mg/L dimethylformamide) and a dilution water control. After 48 hours of exposure there was 100% survival in the control, solvent control and at 0.10 and 1.0 mg/l and 10% survival at 10 mg/L.
The definitive toxicity test with 4-(3-(1-naphthylamino)propyl) morpholine was conducted with 5 concentrations (1.3, 2.2, 3.6, 6.0 and 10 mg/L), a solvent control (95 mg/L dimethylformamaide) and a dilution water control. Ten daphnids were added to each of the two replicate controls and tested concentrations. The test was performed in loosely covered 300 ml glass beakers that each contained 250 ml solution (media depth was approximately 8 cm). Test vessels were randomly arranged for the 48 hour test. A 16:8 hour photoperiod was maintained, loading rate was ≤ 40 daphnids/liter. The number of surviving organisms and the occurence of sublethal effects (immobilization, loss of equilibrium, erratic swimming, loss of reflex, excitability, discolouration or change in behaviour) were determined visually and recorded initially and after 24 and 48 hours. Dead daphnids were removed when observed.
Dissolevd oxygen, pH, conductivity and temperature were measured and recorded daily in each test chamber. Temperature was recorded continuously in a beaker of water incubated among the test vessels. - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 7.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CI - 6.3 - 8.8 mg/l
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CI - 4.4 - 6.0 mg/l
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- No insoluble material was observed at any time during the definitive test. 100% survival with no sublethal effects were noted in the control and solvent control dose groups. Exposure of daphnids, Daphnia magna to 4-(3-(1-naphthylamino)propyl) morpholine resulted in the following effective concentration (EC50's) -
24 hour EC50 - 7.3 mg/l (95% CI: 6.3 - 8.8 mg/l)
48 hour EC50 - 5.1 mg/l (95% CI: 4.4 - 6.0 mg/l).
The 48 hour NOEC was 2.2 mg/l. - Results with reference substance (positive control):
- not applicable
- Reported statistics and error estimates:
- The probit method was used to calculate the 24 and 48 hour median effective concentrations using nominal concentrations and the number of live, mobile daphnids. The no observed effect concentration is the highest concentration of test substance that allows at least 90% survival of exposed organisms and does not cause sublethal effects.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Exposure of daphnids, Daphnia magna to Mortrace MP resulted in the following effective concentration (EC50's) -
24 hour EC50 - 7.3 mg/l (95% CI: 6.3 - 8.8 mg/l)
48 hour EC50 - 5.1 mg/l (95% CI: 4.4 - 6.0 mg/l).
The 48 hour NOEC was 2.2 mg/l. - Executive summary:
The acute toxicity of Mortrace MP was evaluated in daphnid, Daphnia magna under static conditionsn at 20 ± 1 °C with five concentrations of test substance (1.3, 2.2, 3.6, 6.0 and 10 mg/L), a solvent control and a dilution water control (0 mg/l). The dilution water was deionized water collected and adjusted to a hardness of 160 mg/l as CaCO3 and apH less than 8.0. Nominal concentrations of Mortrace MP were used for all calculations. No insoluble material was observed at any time during the definitive test. Exposure of daphnids, Daphnia magna to Mortrace MP resulted in the following effective concentration (EC50's) -
24 hour EC50 - 7.3 mg/l (95% CI: 6.3 - 8.8 mg/l)
48 hour EC50 - 5.1 mg/l (95% CI: 4.4 - 6.0 mg/l).
The 48 hour NOEC was 2.2 mg/l.
Reference
None
Description of key information
In an acute, static, toxicity study with Daphnia magna,the 48-hour EC50 value for 4-(3-(1-naphthylamino)propyl) morpholine was 5.1 mg/L based on nominal concentrations. The 95% confidence interval based on nominal concentrations was 4.4 – 6.0 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 5.1 mg/L
Additional information
One static acute toxicity test with Daphnia magnawas available for 4-(3-(1-naphthylamino)propyl) morpholine. This study was assigned a Klimisch score of 2 based on the lack of analytical measures of test solution concentrations. The 48-hour EC50 value (based on immobility) was reported as 5.1 mg 4-(3-(1-naphthylamino)propyl) morpholine/L with a 95% confidence interval of 4.4 - 6.0 mg/L. This value was based on initial nominal concentrations. Thus, the key parameter for the acute invertebrate endpoint was the EC50 value of 5.1 mg/L.
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