3,4,5-trimethoxycinnamic acid

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1984

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference Type:

publication

Title:

Non-mutagenicity of some wood-related compounds in the bacterial/microsome plate incorporation andmicrosuspension assays

Author:

E Mohtashamipur and K Norpoth

Year:

1984

Bibliographic source:

Int Arch Occup Environ Health (1984) 54: 83-90

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

3,4,5-trimethoxycinnamic acid

EC Number:

201-999-8

EC Name:

3,4,5-trimethoxycinnamic acid

Cas Number:

90-50-6

Molecular formula:

C12H14O5

IUPAC Name:

3-(3,4,5-trimethoxyphenyl)acrylic acid

Test material form:

solid

Details on test material:

yellow

Specific details on test material used for the study:

from Aldrich Co (Steinheim/Albuch, FRG),

Method

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 98

Species / strain 2

Species / strain / cell type:

S. typhimurium TA 100

Metabolic activation:

with

Metabolic activation system:

Liver homogenates were prepared according to the Ames method l4l after induction of liver enzymes by a single i p injection (500 mg/kg) of Clophen A50 (in Mazola oil) into each 30-g male B6C3 F, mouse (for the Ames test)

Test concentrations with justification for top dose:

0, 0.1, 5, 10, 50 and 100 μg/plate. Preliminary toxicity tests had been done to determine the tolerated non-toxic doses for tests.

Vehicle / solvent:

The solvents used were acetone and dimethylsulfoxide, purchased from Merck AG (Schuchardt, FRG).

Controls

Positive controls:

yes

Positive control substance:

2-acetylaminofluorene

benzo(a)pyrene

cyclophosphamide

other: aflatoxin B

Details on test system and experimental conditions:

An overnight culture of each strain in Oxoid broth was used Liver homogenates were prepared according to the Ames method (Ames et al 1977) after induction of liver enzymes by a single i p injection (500 mg/kg) of Clophen A50 (in Mazola oil) into each 30-g male B6C3 F, mouse (for the Ames test) and 200-g male Sprague Dawley/Bio rat (for the fluctuation test) The plate incorporation test-system of Ames in triplicate experiments per dose was followed. The modified (Gatehouse and Delow, 1979) bacterial fluctuation test-system of Green et al 1976 was employed as the second test-system to reevaluate the mutagenic activity of some of these compounds

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 98

Metabolic activation:

with

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not determined

Vehicle controls validity:

not examined

Untreated negative controls validity:

not examined

Positive controls validity:

valid

Test results 2

Species / strain:

S. typhimurium TA 100

Metabolic activation:

with

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not determined

Vehicle controls validity:

not examined

Untreated negative controls validity:

not examined

Positive controls validity:

valid

Applicant's summary and conclusion

Conclusions:

Seven commercially available wood-related compounds have been tested for mutagenicity by the use of the Ames and fluctuation test-systems. All compounds were found to be non-mutagenic