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EC number: 266-867-4 | CAS number: 67674-28-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 Oct - 10 Nov 1978
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- GLP compliance:
- no
- Test type:
- traditional method
- Limit test:
- no
Test material
- Reference substance name:
- Sodium m-[[4-[[p-[bis(2-hydroxyethyl)amino]phenyl]azo]-1-naphthyl]azo]benzenesulphonate
- EC Number:
- 266-867-4
- EC Name:
- Sodium m-[[4-[[p-[bis(2-hydroxyethyl)amino]phenyl]azo]-1-naphthyl]azo]benzenesulphonate
- Cas Number:
- 67674-28-6
- Molecular formula:
- C26H25N5O5S.Na
- IUPAC Name:
- sodium m-[[4-[[p-[bis(2-hydroxyethyl)amino]phenyl]azo]-1-naphthyl]azo]benzenesulphonate
- Test material form:
- solid: particulate/powder
- Details on test material:
- Acid Red 299
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Tif: RAIf (SPF)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation mean: males: 237g; females: 205g
- Housing: Males and females kept segregated in macrolon cages, type 4, (10 animals / cage)
- Diet: ad libitum, rat food
- Water: ad libitum
- Acclimation period: min. 4 days
ENVIRONMENTAL CONDITIONS
- Temperature: 22±1°C
- Humidity: 55±5%
- Photoperiod: 10h light cycle
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- nose only
- Remarks:
- snout and nostrils
- Vehicle:
- air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: Grafix Exaktomat Injector
- Method of holding animals in test chamber: rats kept in separate PVC tubes positioned radially around the exposure chamber
- System of generating particulates/aerosols: the aerosol was generated by injecting two different amounts of the solid test material using the Grafix Exaktomat Injector into an air stream at a rate of 20L/min.
- Method of concentration determination: The concentration was determined 5 times gravimetrically by sampling the test atmosphere through a selectron filter of 50 mm diameter and with a pore size of 0.2 µm at an air flow rate of 10 L/min.
- Method of particle size determination: was measured twice with a 4 stage Cascade Impactor with selectron filters of 25 mm diameter and with a pore size of 0.2 µm at an air flow rate of 17.5 L/min.
- Parameters determination inside the inhalation cylinder: temperature (Therm 2104 contact thermometer); rel, humidity (VASALA humidity indicator HMI 11); oxygen content (DRAEGER E 15 stationary control system)
- Duration of exposure:
- 4 h
- Concentrations:
- 327±40 mg/m³
627±83 mg/m³ - No. of animals per sex per dose:
- 40
- Control animals:
- yes
- Remarks:
- 10 males/10 females
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: body weights were determined prior to exposure and at day 7 and 14. Physical condition and incidence of death were monitored throughout the observation period of 14 days.
- Necropsy of random survivors performed: yes
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 630 mg/m³ air
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- no deaths during the 14 observations days occured
- Clinical signs:
- other: No symptoms were seen in the animals of both concentrations as well as the control group during the exposure and the 14 observation days.
- Body weight:
- see table 1
- Other findings:
- Particle size distribution: airborne particles 60-70 % were smaller than 7µm in diameter.
Any other information on results incl. tables
Table 1: mean body weight
mean Body Weight (g) | Dose (mg/m³) | |||
Time (d) | Sex | 0 (Control) | 327±40 | 627±40 |
1 | male | 237 | 208 | 217 |
1 | female | 205 | 203 | 185 |
7 | male | 260 | 245 | 251 |
7 | female | 212 | 216 | 185 |
14 | male | 290 | 289 | 292 |
14 | female | 221 | 234 | 214 |
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The LC50 of a 4 hour aerosol exposure for rats of both sexes is greater than 630 mg/m³ air, when evaluated for a 14 day posttreatment observation period.
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