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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
partition coefficient
Type of information:
experimental study
Adequacy of study:
key study
Study period:
from 25 April 2016 to 15 May 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report date:
2017

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
EU Method A.8 (Partition Coefficient)
Version / remarks:
2008
Deviations:
no
Qualifier:
according to guideline
Guideline:
OECD Guideline 117 (Partition Coefficient (n-octanol / water), HPLC Method)
Version / remarks:
2004
Deviations:
no
Qualifier:
according to guideline
Guideline:
EPA OPPTS 830.7570 (Partition Coefficient, n-octanol / H2O, Estimation by Liquid Chromatography)
Version / remarks:
1996
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Type of method:
HPLC method
Partition coefficient type:
octanol-water

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Details on test material:
- Appearance: yellow/brown powder
- Storage conditions: in refrigerator (2-8°C)

Results and discussion

Partition coefficientopen allclose all
Key result
Type:
log Pow
Partition coefficient:
3.4
Temp.:
20 °C
Remarks on result:
other: unbuffered mobile phase
Key result
Type:
Pow
Partition coefficient:
2 400
Temp.:
20 °C
Remarks on result:
other: unbuffered mobile phase
Details on results:
- Retention times of reference substances used for calibration: see table below
- Details of fitted regression line: log k' = 0.490 x log Pow – 0.743 (r = 0.991, n=12)
- Graph of regression line and chromatograms: see attachment
- The chromatogram of the test item showed one substance peak
- Average retention data for test item: see table below
- The value of log Pow obtained from duplicate measurements was within ± 0.1 log units.

Any other information on results incl. tables

Table: Results Partition coefficient n-octanol-water (HPLC method)

 

Substance

tr1 [min]

tr2
[min]

mean tr
(n=2)

log Pow

Pow

Formamide (t0)

0.977

0.926

0.952

 

 

 

 

 

 

 

 

2-Butanone

1.099

1.303

 

0.3

 

Nitrobenzene

2.377

2.310

 

1.9

 

Toluene

5.493

5.359

 

2.7

 

1,4-Dichlorobenzene

9.342

9.096

 

3.4

 

Biphenyl

16.581

16.023

 

4.0

 

1,2,4-Trichlorobenzene

18.638

17.964

 

4.2

 

 

 

 

 

 

 

Test item

8.896

8.697

8.797

3.4

2.4E03

Remark: the log Pow values of the reference substances are according to OECD TG 117.

Applicant's summary and conclusion

Conclusions:
The log Pow of the substance was determined to be 3.4.

Executive summary:

The log Pow of the substance was determined using the HPLC method in a GLP study according to EC A.8, OECD 117 and OPPTS 830.7570. The mobile phase was 60/40 (v/v) methanol/water and the wavelength of detection was 210 nm. Formamide was used as unretained substance. Duplicate injections of unretained substance, reference substances and test item. The chromatogram of the test item showed one peak. The mean (n=2) log Pow was determined to be 3.4 (Pow= 2.4E03).