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EC number: 268-516-0
CAS number: 68110-27-0
The test substance, Reactive Yellow 85, was non mutagenic for all the Salmonella typhimurium as well as Escherichia coli strains with as well as without metabolic activation.
The test substance Reactive Yellow 85 was assayed for the mutagenicity by the Bacterial Reverse Mutation Test. The performed test was based on EU method B.13/14 Mutagenicity – Reverse mutation test using bacteria, which is analogous to the OECD Test Guideline No. 471.
Four indicator Salmonella typhimurium strains TA 98, TA 100, TA 1535, TA 1537 and one indicator Escherichia coli WP2 uvrA strain were used.
The test substance was diluted in water for injection and assayed in doses of 50 - 5000 ug per plate, which were applied to plates in volume of 0.1 mL.
The first mutagenicity experiments were performed without and with metabolic activation using a supernatant of rat liver (30 μL or 100 μL per plate) and a mixture of cofactors by the plate incorporation test with a dose range of 50 – 5000 ug per plate.
To modify experimental conditions and to increase the sensitivity of the assay, the second mutagenicity experiments were performed with pre-incubation and concentrations were shifted towards expected mutagenic doses to obtain a dose-response.
The concurrent positive controls verified the sensitivity of the assay and the metabolising activity of the liver preparations. Mean revertant colony counts for the vehicle controls were within the current historical control range for the laboratory.
In the arrangement given above, the test substance, Reactive Yellow 85, was non-mutagenic for all the used tester strains without as well as with metabolic activation.
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