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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

The test item attained 7 % biodegradation after 28 days and 21 % biodegradation after 35 days and therefore cannot be considered to be readily biodegradable (OECD 301 B).

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

GUIDELINE

A study was performed to assess the ready biodegradability of the test item in an aerobic aqueous medium. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No. 301B, "Ready Biodegradability; CO2 Evolution Test" referenced as Method C.4-C of Commission Regulation (EC) No. 440/2008 and US EPA Fate, Transport, and Transformation Test Guidelines OCSPP 835.3110 (Paragraph (m)).

 

METHODS

The test item, at a concentration of 10 mg carbon/L, was exposed to activated sewage sludge micro organisms with mineral medium in sealed culture vessels in the dark at temperatures of between 20 and 24 °C for 35 days. At the request of the Sponsor the study was extended from 28 to 35 days. An approach endorsed by the International Standards Organisation (ISO 10634, (1995)) and the published literature (Handley et al, 2002) is to adsorb the test item onto an inert support prior to dispersion in the test vessels. Using this method ensures that all the test item is added into the test vessels and that none is lost through adhering to glassware used to weigh the test item out in. The biodegradation of the test item was assessed by the determination of carbon dioxide produced. Control solutions with inoculum and the reference item, sodium benzoate, together with a toxicity control were used for validation purposes.

 

RESULTS

The test item attained 7 % biodegradation after 28 days and 21 % biodegradation after 35 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301 B.