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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

A valid, guideline-conform CO2 evolution study according to OECD 310 B was conducted under GLP. A biodegradation degree of 50-60 % CO2/ThCO2 was observed after an exposure period of 28 days. The test substance therefore was not readily but inherently biodegradable under the conditions of this test.

Key value for chemical safety assessment

Biodegradation in water:
inherently biodegradable

Additional information

The carbon dioxide evolution test (former Sturm test) is a static method to evaluate the ultimate aerobic biodegradability of a test substance in water. The test substance, a defined inorganic medium and a non pre-adapted inoculum (e.g. activated sludge or effluent of a municipal waste water treatment plant) were incubated and aerated at up to 28 days. The biologically produced carbon dioxide is trapped and measured. The biodegradation degree is expressed as produced carbon dioxide in relation to the calculated theoretical carbon dioxide value (ThCO2) using the initial concentration of total organic carbon of the test substance (TOC).

Biodegradation degree: 50-60 % CO2/ThCO2 after an exposure period of 28 days (mean value from two single test assays) . The test substance was partly or moderately biodegradable under the conditions of this test.

Evaluation in accordance to criteria of the OECD Test Guideline: The test substance was not readily but inherently biodegradable under the conditions of this test.