Registration Dossier
Registration Dossier
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EC number: 229-880-6 | CAS number: 6805-41-0
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Escin
- EC Number:
- 229-880-6
- EC Name:
- Escin
- Cas Number:
- 6805-41-0
- Molecular formula:
- C110H172O48
- IUPAC Name:
- (2S,3S,4S,5R,6R)-6-{[(3S,4S,4aR,6aR,6bS,8R,8aR,9R,10R,12aS,14aR,14bR)-9-(acetyloxy)-8-hydroxy-4,8a-bis(hydroxymethyl)-4,6a,6b,11,11,14b-hexamethyl-10-[(2-methylbutanoyl)oxy]-1,2,3,4,4a,5,6,6a,6b,7,8,8a,9,10,11,12,12a,14,14a,14b-icosahydropicen-3-yl]oxy}-4-hydroxy-3,5-bis({[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy})oxane-2-carboxylic acid; (2S,3S,4S,5R,6R)-6-{[(3S,4S,4aR,6aR,6bS,8R,8aR,9R,10R,12aS,14aR,14bR)-9-(acetyloxy)-8-hydroxy-4,8a-bis(hydroxymethyl)-4,6a,6b,11,11,14b-hexamethyl-10-{[(2E)-2-methylbut-2-enoyl]oxy}-1,2,3,4,4a,5,6,6a,6b,7,8,8a,9,10,11,12,12a,14,14a,14b-icosahydropicen-3-yl]oxy}-4-hydroxy-3,5-bis({[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy})oxane-2-carboxylic acid
- Test material form:
- solid
Constituent 1
Test solutions
- Vehicle:
- yes
- Remarks:
- OECD medium
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Study design
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21.5-22.9°C
- pH:
- 7.64-8.88
- Nominal and measured concentrations:
- 0.30, 0.95, 3.05, 9.77, 31.25 and 100 mg/L
- Details on test conditions:
- Light intensity: The light intensity was checked and recorded at the start of the test. The algal culture flask were continuosly illuminated. The average light intensity measured at the position occupied by algal culture flask during the test was 8030 lux, which was ensured with fluorescent lamps. The differences in light intensity between the test vessels did not exceed +- 15% and therefore provided equal conditions for each test vessel.
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 10.27 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 22.95 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 106.88 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 2.48 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 4.95 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 18.62 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Results with reference substance (positive control):
- Growth
The 72 h ErC50 : 0.82 mg/L (95% confidence limits: 0.57 - 1.25 mg/L)
Yield
The 72 h EyC50 : 0.51 mg/L (95% confidence limits: 0.45 - 0.57 mg/L)
Any other information on results incl. tables
Growth rate. Accordingly the 72 h NOEC based on growth rate was determined to be: 3.05 mg/L
Accordingly the 72 h ErC50 value was determined to be: 106.88 mg/L
Yield
Accordingly the 72 h NOEC based on yield rate was determined to be: 3.05 mg/L
Accordingly the 72 h EyC50 value was determined to be: 4.95 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Re: results
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