Registration Dossier

Administrative data

Endpoint:
partition coefficient
Type of information:
experimental study
Adequacy of study:
key study
Study period:
7 July 1993 - 28 September 1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report Date:
1995

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method A.8 (Partition Coefficient - Shake Flask Method)
Deviations:
no
GLP compliance:
yes
Type of method:
flask method
Partition coefficient type:
octanol-water

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Test material: Simazine (pure)
- Appearance: White powder
- Batch No.of test material: Ref 766
- Expiration date of the batch: Two years from date of manufacture
- Purity: 99.5%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: In the dark at 4°C

Study design

Analytical method:
high-performance liquid chromatography

Results and discussion

Partition coefficient
Type:
log Pow
Partition coefficient:
2.18
Temp.:
20 °C
Remarks on result:
other: Mean result
Details on results:
Calibration data gave a slope of 557606, an intercept of -1126.8 and a correlation coefficient of 0.99861, showing the detector response to be linear in the concentration range employed.

No peak was observed at the retention volume of the test substance in any of the blanks showing that the analytical procedure was free from interferences.

The mean value for the partition coefficient from all determinations was:

P = 151.46
Log P = 2.18

Standard deviation of individual P values about their mean is 17.303.

The range of log P values obtained was 0.12 units - within the repeatability criterion of ±0.3 log units.

Recoveries in the range 98.1 - 104.8% were obtained in the mass balance, hence accounting for all of the test substance.

Applicant's summary and conclusion

Conclusions:
The partition coefficient (n-octanol/ water) of simazine (pure) is:

log P = 2.18 at 20°C