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Ecotoxicological information

Toxicity to microorganisms

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Description of key information

Key value for chemical safety assessment

EC50 for microorganisms:
380 mg/L
EC10 or NOEC for microorganisms:
140 mg/L

Additional information

The toxicity of Methyl salicylate to bacteria was tested (Rhône-Poulenc Industrialisation - Centre de Décines, 1992) in a growth inhibition test using Pseudomonas putida, according to the draft standard ISO / CD 10712 (1990 -10 -10). EC values were determined by comparing the turbidity (standard ISO 7027 / 1984 -07 -01) of the test and control cultures after 16 ± 1 hours incubation. The EC10 and EC50 values were 140 and 380 mg/L respectively.

Since the product is hydrolysable, it was verified from Methyl salicylate solutions beforehand maintained for 120 hours under magnetic stirring at 24°C and in the dark, that their toxicity decreased slightly. In this case, EC10 and EC50 values were 162 and 500 mg/L respectively.

The EC10 and EC50 values of 3,5 -dichlorophenol were 16 and 22 mg/L respectively. These EC values are within the confidence limits selected by the subcommittee ISO/TC 147/SC5.