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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Description of key information

Key value for chemical safety assessment

EC50 for microorganisms:
380 mg/L
EC10 or NOEC for microorganisms:
140 mg/L

Additional information

The toxicity of Methyl salicylate to bacteria was tested (Rhône-Poulenc Industrialisation - Centre de Décines, 1992) in a growth inhibition test using Pseudomonas putida, according to the draft standard ISO / CD 10712 (1990 -10 -10). EC values were determined by comparing the turbidity (standard ISO 7027 / 1984 -07 -01) of the test and control cultures after 16 ± 1 hours incubation. The EC10 and EC50 values were 140 and 380 mg/L respectively.

Since the product is hydrolysable, it was verified from Methyl salicylate solutions beforehand maintained for 120 hours under magnetic stirring at 24°C and in the dark, that their toxicity decreased slightly. In this case, EC10 and EC50 values were 162 and 500 mg/L respectively.

The EC10 and EC50 values of 3,5 -dichlorophenol were 16 and 22 mg/L respectively. These EC values are within the confidence limits selected by the subcommittee ISO/TC 147/SC5.