Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to other aquatic vertebrates
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Cross-reference
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
publication
Title:
Acute toxicity of substituted phenols to Rana japonica tadpoles and mechanism-based quantitative structure-activity relationship (QSAR) study
Author:
Wang X., Dong Y., Wang L. & Han S.
Year:
2001
Bibliographic source:
Chemosphere, 44 (3), 447-455

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Acute toxicity of Methyl salicylate to R. japonica tadpoles was determined post 12 -h and 24 -h semi-static exposure. Thus, a six-step concentration series in geometric grade ranging from no effect to 100% lethal concentration were set after range finding experiments. Ten tadpoles were employed for each test and placed in a 1000 ml pyrex beaker containing 500 ml test solution or aerated chloride-free water (control). All experiments were performed with three replicates of each treatment group and unexposed controls. Mortality was recorded and 12-h and 24-h LC50, based on nominal concentrations, was calculated.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl salicylate
EC Number:
204-317-7
EC Name:
Methyl salicylate
Cas Number:
119-36-8
Molecular formula:
C8H8O3
IUPAC Name:
methyl salicylate
Details on test material:
- Name of test material (as cited in study report): 2-Hydroxyl, methyl benzoate
- Supplier: Methyl salicylate was provided by Department of Chemistry at Nanjing University.
- Analytical purity: This substance was of sufficient purity (analytical purity) and further purification was not necessary.

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
no data

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION:
- Method: Aerated chloride-free water was used for preparing the stock solutions of chemicals and test dilution.
- Controls: The aerated chloride-free water without test compounds served as the control.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no data
- Concentration of vehicle in test medium (stock solution and final test solution): no data

Test organisms

Test organisms (species):
other: Rana japonica
Details on test organisms:
TEST ORGANISM
- Common name: frog (tadpole)
- Strain: Rana japonica
- Source: Naturally fertilized egg masses of R. japonica were collected from a permanent pond in Nanjing, where R. japonica were exclusively bred.
- Age/weight/length at study initiation: Thirty-day-old (stage 30 to stage 32, Gosner (1960)) healthy individuals of similar size and weight were employed for the test. The average body length and body weight were 2.5 ± 0.1 cm and 0.09 ± 0.01 g, respectively.
- Method of breeding: Eggs and the resulting tadpoles were cultured in aerated stream water (Cl- 3.40 mg/l, pH 7.74, dissolved oxygen concentration 9.6 mg/l, Na+ 2.35 mg/l, Ca2+ 48 mg/l) in the laboratory at room temperature (22-25°C). Tadpoles were fed an excess of rinsed, salt free canned spinach daily and water was renewed every other day. Feeding was halted 24h prior to testing.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Post exposure observation period:
no data

Test conditions

Hardness:
no data
Test temperature:
All exposures took place at water temperature 22-25°C.
pH:
The pH of test solution, measured by a PHS-3C pH-Meter, was 6.55.
Dissolved oxygen:
no data
Salinity:
no data
Nominal and measured concentrations:
no data
Details on test conditions:
TEST SYSTEM
- Test vessel: 1000 ml pyrex beakers containing 500 ml test solution or aerated chloride-free water.
- No. of organisms per vessel: Ten tadpoles were employed for each test.
- No. of vessels per concentration (replicates): All experiments were performed with three replicates of each treatment group.
- No. of vessels per control (replicates): All experiments were performed with three replicates.
- No. of vessels per vehicle control (replicates): no data
- Renewal rate of test solution (frequency/flow rate): Solutions and the control were renewed every 6 h to achieve semi-static exposure.

OTHER TEST CONDITIONS
- Adjustment of pH: The pH of test solution, measured by a PHS-3C pH-Meter, was 6.55.
- Photoperiod: 14:10 (L:D)
- Temperature: All exposures took place at water temperature 22-25°C in a controlled environmental chamber.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
Mortality was recorded and LC50 based on nominal concentrations was calculated.

TEST CONCENTRATIONS
- Range finding study: no data
- Test concentrations: A six-step concentration series in geometric grade ranging from no effect to 100% lethal concentration were set after range finding experiments.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
12 h
Dose descriptor:
LC50
Effect conc.:
3.315 mmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
3.303 mmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Mortality of control: The test was terminated on condition that mortality in controls exceeded 10%.
- 12-h and 24-h LC50 were respectively 3.315 and 3.303 mmol/L, ie 504.38 and 502.55 mg/L.
Results with reference substance (positive control):
no data
Reported statistics and error estimates:
Logarithms of inverse of median lethal concentrations after 12-h and 24-h exposure, expressed as 12 h-LC50 (mmol/l) and 24 h-LC50 (mmol/l), were calculated for each compound as toxicity using nonlinear regression in STATISTICA for Windows software (1995).

Applicant's summary and conclusion

Conclusions:
Acute toxicity of Methyl salicylate to R. japonica tadpoles was determined post 12 -h and 24 -h semi-static exposure. 12 -h and 24 -h LC50 were respectively 3.315 and 3.303 mmol/l, ie 504.38 and 502.55 mg/l.