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EC number: 413-110-2 | CAS number: 135861-56-2
The analytical detection system was found to have acceptable linearity. The analytical procedure had acceptable recoveries of test material in test medium. A method of analysis was validated and proven to be suitable for use. The test material was stable in the test medium for the duration of the test period.
Table 1: Summary of findings following exposure of Daphnia magna for 21 days
Nominal concentration (mg/L)
% survival of P1
Number of live young
Number of dead young
Number of unhatched eggs
Per female (cumulative)
Table 2: Mean length of the surviving daphnids of the P1 generation
Day 21 (mm)
A study was performed to assess the effect of the test material on the reproduction of Daphnia magna over a 21-day period. The method followed that described in the OECD Guidelines for Testing of Chemicals No 211 (1998), referenced as Method C.20 of Commission Directive 2001/59/EC.
The 14 and 21-Day EC50 (immobilisation) values, based on nominal test concentrations, for the parental Daphnia generation (P1) were estimated to be greater than 0.048 mg/L.
The 21-Day EC50 (reproduction) value based on nominal test concentrations was estimated to be greater than 0.048 mg/L.
The LOEC based on nominal test concentrations was considered to be greater than 0.048 mg/L on the basis that at this test concentration no significant mortalities (immobilisation) were observed in the parental generation (P1) and that there were no significant differences (P≥0.05) between the solvent control and the 0.048 mg/L test group in terms of numbers of live young produced per adult by Day 21.
The NOEC based on nominal test concentrations was considered to be 0.048 mg/L on the basis that at this test concentration no significant mortalities (immobilisation) were observed in the parental generation (P1) and that there were no significant differences (P≥0.05) between the solvent control and the 0.048 mg/L test group in terms of numbers of live young produced per adult by Day 21.
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