Registration Dossier

Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
6th December 2001 to 18th December 2001
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report Date:
2002

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Version / remarks:
HPLC Screening method, Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001
Deviations:
no
GLP compliance:
yes (incl. certificate)
Type of method:
HPLC estimation method
Media:
soil/sewage sludge

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Sponsor's identification: Experimental 11334-18

Data relating to the identity, purity and stability of the test material are the responsibility of the Sponsor.
Radiolabelling:
no

Study design

Test temperature:
Column temperature: 40 °C

HPLC method

Details on study design: HPLC method:
EQUIPMENT
- Apparatus: HPLC
- Type: Agilent Technologies 1050, incorporating autosampler and workstation
- Type, material and dimension of analytical (guard) column: Luna CN (250 x 4.6 mm id)
- Detection system: UV detector (wavelength for dead time and reference standards: 210 nm; wavelength for sample: 268 nm)

MOBILE PHASES
- Type: Methanol : Water (55:45 v/v); Flow rate: 1 mL/min
- Experiments with additives carried out on separate columns: No
- pH: 6.4 (the pH was not corrected)
- Solutes for dissolving test and reference substances: Not applicable

DETERMINATION OF DEAD TIME
- Method: The dead time was determined by measuring the retention time of formamide (664 mg/L solution in methanol).


REFERENCE SUBSTANCES
- Identity: The solutions of reference standards (detailed in Table 1) were prepared in methanol.


DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: The test material (0.2470 g) was diluted to 100 mL with dimethylsulfoxide. The injection volume was 5 µL.
- Quantity of reference substances: The concentrations of the reference standards are given in Table 1. The injection volume was 5 µL.
- Intervals of calibration: Not specified


REPETITIONS
- Number of determinations: The sample, formamide and reference standard solutions were injected in duplicate.


EVALUATION
- Calculation of capacity factors k': The capacity factor was determined using:
k = (t^t - t^0)/t^0
where: t^t = retention time (min); t^0 = dead time (min)
- Calculation of retention times: Not specified
- Determination of the log Koc value: The log Koc value was determined with reference to the calibration curve.

Batch equilibrium or other method

Analytical monitoring:
no
Details on sampling:
Not applicable
Details on matrix:
Not applicable
Details on test conditions:
Not applicable
Computational methods:
No dat available

Results and discussion

Adsorption coefficientopen allclose all
Type:
Koc
Value:
1 610
Type:
log Koc
Value:
3.21

Results: HPLC method

Details on results (HPLC method):
- Retention times of reference substances used for calibration: The mean retention time of formamide was 3.268 mins. The retention times, capacity factors and log Koc values for the reference standards are given in Table 2.
- Details of fitted regression line (log k' vs. log Koc): r = 0.947
- Average retention data for test substance: The retention times, capacity factors and log Koc values for the test samples are shown in Table 3. Average retention time was 4.413 mins.

Results: Batch equilibrium or other method

Adsorption and desorption constants:
Not applicable
Recovery of test material:
Not applicable
Concentration of test substance at end of adsorption equilibration period:
Not applicable
Concentration of test substance at end of desorption equilibration period:
Not applicable
Transformation products:
not measured
Details on results (Batch equilibrium method):
Not applicable
Statistics:
Not applicable

Any other information on results incl. tables

Table 2: Results for reference standards

Standard

Mean retention time (mins)

Capacity factor (k)

Log10k

Log10Koc

Acetanilide

3.424

0.04744

-1.321

1.25

Phenol

3.537

0.08231

-1.085

1.32

Atrazine

3.785

0.158

-0.801

1.81

Isoproturon

3.987

0.220

-0.658

1.86

Triadimenol

4.082

0.249

-0.604

2.40

Naphthalene

4.542

0.390

-0.409

2.75

Endosulfan-diol

4.248

0.300

-0.523

3.02

α-endosulfan

5.407

0.654

-0.184

4.09

Phenanthrene

5.483

0.678

-0.169

4.09

Diclofop-methyl

5.232

0.601

-0.221

4.20

DDT

7.753

1.372

-0.137

5.63

Table 3: Results for test samples

Injection

Retention time (mins)

Capacity factor (k)

Log10k

Log10Koc

1

4.408

0.349

-0.457

3.20

2

4.417

0.352

-0.454

3.21

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The adsorption coefficient (Koc) of the test material has been determined to be 1610, log Koc 3.21.
Executive summary:

The adsorption coefficient (Koc) of the test material has been determined to be 1610, log Koc 3.21. The study was performed in accordance with OECD Guideline No. 121.