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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

The definitive semi-static test No. 1 was performed under semi-static conditions (with the renewal of the test solutions after 48 hours) with a range of the test substance nominal concentrations of 40, 48, 58, 69, 83 and 100 mg· L-1. Observed mortality of fish in this definitive semi-static test No. I did not correspond to the mortality observed in the preliminary test No.2, therefore the definitive semi-static test No. 2 was performed. The definitive semistatic test No. 2 was performed with a range of the test substance nominal concentrations of 31 , 34, 37, 41, 45 and 50 mg· L-1.  After renewal of the test solutions (after 48 hours) mortality of all fi sh in the all tested concentrations was observed.

Based on the results of fi sh mortality observed in the preliminary and the definitive semistatic tests, the testing was terminated.

Analytical determination of concentrations of the double hydrolysed product indicate that the stock solutions prepared for the tests have different composition and these differences in composition cause different mortality of the fish.

During all testing, there were only two consistent observations:

In the test concentration of 100 mg· L-1 it was observed always the mortality of all fi sh and at the concentration of 10 mg· L-1 no mortality in the preliminary tests was observed.

Considering the result of all executed tests it could be only estimated that the value of the 96 hour-LCso will probably lie in the interval 10 -100 mg·L·1 of nominal concentration

Key value for chemical safety assessment

LC50 for freshwater fish:
10 mg/L

Additional information