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EC number: 279-506-0 | CAS number: 80584-92-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989-06-21
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Non GLP study. Deviations from the updated OECD-guideline (adopted 2010): The coefficient of analysis of the control was not reported and a reduced number of replicates in the treatment groups were used. Settled sludge was used. Direct weighted amounts were added to the test medium instead of a stock solution.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- Non GLP study. The coefficient of analysis of the control was not reported and a reduced number of replicates in the treatment groups were used. Settled sludge was used. Direct weighted amounts were added to the test medium instead of a stock solution.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Differential loading: 20.21, 6.51, 2.66, 0.93 rsp. 0.32 mg of the test substance were weighed and added to the test medium. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Pretreatment: The sludge was separated from the aqueous layer by settling instead of centrifugation.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 +/- 2 °C
- Nominal and measured concentrations:
- 101, 32.5, 13.3, 4.7 and 1.6 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL BOD flasks with glas inlet
- No. of vessels per concentration: one
- No. of vessels per control: two blank and three reference controls
- Biomass loading rate: 1.7- 1.8 g/L
TEST MEDIUM / WATER PARAMETERS
- according to the guideline
EFFECT PARAMETERS MEASURED :
inhibition - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 101 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: IC20
- Effect conc.:
- > 101 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: IC80
- Effect conc.:
- > 101 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: IC50 > 100 mg/L - Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Results published in peer-reviewed journal; study performed according to guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solutions (0.5 to 5.0 g/L) of the test chemical were prepared in deionized water. When necessary, the stock solutions were adjusted to pH 7.5 ± 0.5 by the addition of 1N H2SO4 or 1N NaOH as required - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source of inoculum: municipal wastewater treatment plant (sampling: one day prior to test start)
- Preparation of inoculum for exposure: after sampling of activated sludge, solids were allowed to settle, waste liquor was discarded, solids diluted to 8.5 L with deionised water, aerating (0.5 L/min), concentration of mixed liquor suspended solids (MLSS) was determined; again solids were allowed to settle, liquor was discarded, sludge was washed three times with with deionised water. MLSS adjusted to 4000±400 mg dry weight per liter, aerated (0.5 L/min), incubated at 21 °C; synthetic sewage stock solution (50 mL/L; similar to OECD, but increased K2HPO4 concentration)
- Initial biomass concentration: MLSS adjusted to 4000±400 mg dry weight per liter - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 21 °C
- pH:
- 7.5±0.5
- Nominal and measured concentrations:
- Nominal: no data
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 1L-bottle, final volume 500 mL
- Aeration: 0.5 - 1.0 L/min
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water
OTHER TEST CONDITIONS
- Adjustment of pH: yes, 7.5 ± 0.5
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Respiration rate: Orion model 97.08 polarographic oxygen electrode and Orion model 701 Ionanalyzer (respiration rate determined ove 5 minute period; mg O2/L/min) - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorphenol
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The synthetic sewage stock solution was prepared similar to OECD 209, but contained a higher K2HPO4 concentration. This concentration was modified after performance of a number of preliminary inhibition studies. It was observed that the pH of a test reaction often changed during the 3-hour incubation period. Thus, decreases in the respiration rate may have been due to a combination of both the effects of pH and toxicity of the test chemical. As a consequence, the buffering capacity of the synthetic sewage nutrient medium was increased to prevent changes in pH of reaction mixtures.
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: IC50 for 3,5-dichlorphenol: 12.2 ± 2.2 mg/L (18% variance) (municipal sewage) - Reported statistics and error estimates:
- Inhibition data were analyzed using Thompson's method of moving averages to estimate IC50 values. The experimental data were also analyzed using a probit-transformation model similar to that described by Larson and Schaeffer (1982). A nonlinear curve-fitting program (Procedure NLIN of SAS) was used to estimate IC50 values and associated 95% confidence intervals.
- Validity criteria fulfilled:
- yes
Referenceopen allclose all
Description of key information
The compound consists of a triazine part and an amine part. Under environmental conditions it is present in its dissociated form. Therefore, separate assessments were conducted for the triazine and the amine part, respectively.
CAS 80584-91-4: The inhibition of the degradation activity of activated sludge is not anticipated when introduced to biological treatment plants in appropriate low concentrations.
CAS 102-71-6: The inhibition of the degradation activity of activated sludge is not anticipated when introduced to biological treatment plants in appropriate low concentrations.
Key value for chemical safety assessment
Additional information
The compound consists of a triazine part and an amine part. Under environmental conditions it is present in its dissociated form. Therefore, separate assessments were conducted for the triazine and the amine part, respectively.
CAS 80584-91-4
The respiration inhibition rate was assessed in a 3 hour respiration inhibition test in accordance to OECD guideline 209. The test item was exposed to activated sludge at nominal concentrations of 101, 32.5, 13.3 4.7 and 1.6 mg/L. The IC50 was determined to be > 101 mg/L.
CAS 102-71-6
Concerning the acute toxicity to microorganisms a test performed by Klecka et al. (1985) according to OECD TG 209 was identified as key study. Using activated, predominantly municipal sludge as inoculum in a static procedure an EC50 of > 1000 mg/l after 180 minutes of exposure was calculated.
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