Reaction mass of 1-Propanaminium, 2,3-dihydroxy-N,N-dimethyl-N-[3-[(1-oxododecyl)amino]propyl]-, chloride and 1-Propanaminium, 2,3-dihydroxy-N,N-dimethyl-N-[3-[(1-oxotetradecyl)amino]propyl]- , chloride

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

20 sept 2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Cocamidopropyl PG-dimonium chloride

Cas Number:

136920-08-6

Molecular formula:

C20-30H43-63ClN2O2 range

IUPAC Name:

Cocamidopropyl PG-dimonium chloride

Test material form:

other: aqeuous solution

Details on test material:

Cocamidopropyl PG-dimonium chloride
CAS# 136920-08-6
Lot# CJ9157
% active: 35%
date: 11/19/2003

Specific details on test material used for the study:

storage conditions: room temperature, in the dark

Sampling and analysis

Details on sampling:

The test material was dissolved directly in the culture medium

Test solutions

Vehicle:

no

Details on test solutions:

The test material was dissolved directly in the culture medium.
An amount of 160 mg of test material was dissolved in culture medium and the volume adjusted to 500 mL to give a 320 mg/L stock solution from which a series of dilutions was made to give further stock solutions of 32, 16, 8, 4, and 2 mg/L. An aliquot (250mL) of each of the 2, 4, 8, 16, and 32 mg/L stock solutions was separately mixed with algal suspension (250mL) to give the required test concentrations of 1, 2, 4, 8, 16 mg/L.

The stock solutions and each of the prepared concentrations were inverted several times to ensure adequate mixing and homogeneity.

The concentration, homogeneity, and stability of the test material in the test preparations were not determined at the request of the Sponsor.

Test organisms

Test organisms (species):

other: scenedesmus Subspicatus

Details on test organisms:

strain CCAP 276/20. Liquid cultures of Scenedesmus Subspicatus were obtained from the culture collection of algae and protozoa (CCAP), Institute of Freshwater Ecology, The Ferry House, Far Sawrey, Ambleside, Cumbria. Cultures were maintained in the lab by the periodic replenishment of culture medium. The culture was maintained in the lab at a temperature of 21C under continuous illumination (intensity approximately 7000 lux) and constant aeration

Test conditions

Test temperature:

24 +/-1 C

pH:

t=0 pH=7.7
t=72hrs pH=8.1

Nominal and measured concentrations:

test concentrations: 1, 2, 4, 8, and 16 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

3.7 mg/L

Basis for effect:

biomass

Remarks on result:

other: 95% confidence limits

Remarks:

3 - 4 mg/L

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

12 mg/L

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

1 mg/L

Applicant's summary and conclusion

Conclusions:

The effect of the test material on the growth of Scenedesmus subspicatus has been investigated over a 72-hr period and gave EC50 (72hr / biomass) value of 3.7 mg/L; 95% confidence limits 3.0-4.6 mh/L and EC50 (72hr growth rate) value of 12 mg/L. The No Oserved Effect Concentration at 72 hrs was 1.0 mg/L

Executive summary:

Using test method OECD 201, the effect of the test material on the growth of Scenedesmus subspicatus has been investigated over a 72-hr period and gave EC50 (72hr / biomass) value of 3.7 mg/L; 95% confidence limits 3.0-4.6 mh/L and EC50 (72hr growth rate) value of 12 mg/L. The No Oserved Effect Concentration at 72 hrs was 1.0 mg/L.