Vetiveria zizanioides, ext., acetylated

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 835.3110 (Ready Biodegradability)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

rel-((3aR,6R)-7,7-dimethyl-8-methyleneoctahydro-1H-3a,6-methanoazulen-3-yl)methyl acetate

Molecular formula:

C17H26O2

IUPAC Name:

rel-((3aR,6R)-7,7-dimethyl-8-methyleneoctahydro-1H-3a,6-methanoazulen-3-yl)methyl acetate

Constituent 2

Reference substance name:

2-(8,8a-dimethyl-3,4,6,7,8,8a-hexahydronaphthalen-2(1H)-ylidene)propyl acetate

Molecular formula:

C17H26O2

IUPAC Name:

2-(8,8a-dimethyl-3,4,6,7,8,8a-hexahydronaphthalen-2(1H)-ylidene)propyl acetate

Constituent 3

Reference substance name:

rel-(8R,8aS)-8,8a-dimethyl-2-(propan-2-ylidene)-1,2,3,7,8,8a-hexahydronaphthalene

Molecular formula:

C15H22

IUPAC Name:

rel-(8R,8aS)-8,8a-dimethyl-2-(propan-2-ylidene)-1,2,3,7,8,8a-hexahydronaphthalene

Constituent 4

Reference substance name:

rel-(5R,10R)-6,10-dimethyl-2-(propan-2-ylidene)spiro[4.5]dec-6-en-8-one

Cas Number:

22196-46-9

Molecular formula:

C15H22O

IUPAC Name:

rel-(5R,10R)-6,10-dimethyl-2-(propan-2-ylidene)spiro[4.5]dec-6-en-8-one

Constituent 5

Reference substance name:

rel-(4R,4aS)-4,4a-dimethyl-6-(propan-2-ylidene)-4,4a,5,6,7,8-hexahydronaphthalen-2(3H)-one

Cas Number:

16981-90-1

Molecular formula:

C15H22O

IUPAC Name:

rel-(4R,4aS)-4,4a-dimethyl-6-(propan-2-ylidene)-4,4a,5,6,7,8-hexahydronaphthalen-2(3H)-one

Constituent 6

Reference substance name:

rel-(1R,8aS)-1,8a-dimethyl-7-(propan-2-ylidene)-1,2,3,7,8,8a-hexahydronaphthalene

Molecular formula:

C15H22

IUPAC Name:

rel-(1R,8aS)-1,8a-dimethyl-7-(propan-2-ylidene)-1,2,3,7,8,8a-hexahydronaphthalene

Constituent 7

Reference substance name:

2-methoxy-4-prop-1-enylphenyl acetate

EC Number:

202-236-1

EC Name:

2-methoxy-4-prop-1-enylphenyl acetate

Cas Number:

93-29-8

Molecular formula:

C12H14O3

IUPAC Name:

2-methoxy-4-(prop-1-en-1-yl)phenyl acetate

Constituent 8

Reference substance name:

rel-(1R,4aS,8aR)-1,4a-dimethyl-7-(prop-1-en-2-yl)-1,2,3,4,4a,5,6,8a-octahydronaphthalene

Molecular formula:

C15H24

IUPAC Name:

rel-(1R,4aS,8aR)-1,4a-dimethyl-7-(prop-1-en-2-yl)-1,2,3,4,4a,5,6,8a-octahydronaphthalene

Constituent 9

Reference substance name:

rel-(3aR,6R,8aR)-7,7-dimethyl-8-methylenehexahydro-1H-3a,6-methanoazulen-3(2H)-one

Cas Number:

64550-95-4

Molecular formula:

C14H20O

IUPAC Name:

rel-(3aR,6R,8aR)-7,7-dimethyl-8-methylenehexahydro-1H-3a,6-methanoazulen-3(2H)-one

Constituent 10

Reference substance name:

rel-(1R,4aS,8aR)-1-isopropyl-4,7-dimethyl-1,2,4a,5,6,8a-hexahydronaphthalene

Cas Number:

31983-21-8

Molecular formula:

C15H24

IUPAC Name:

rel-(1R,4aS,8aR)-1-isopropyl-4,7-dimethyl-1,2,4a,5,6,8a-hexahydronaphthalene

Constituent 11

Reference substance name:

6-isopropyl-4,8a-dimethyl-1,2,3,7,8,8a-hexahydronaphthalene

Cas Number:

473-14-3

Molecular formula:

C15H24

IUPAC Name:

6-isopropyl-4,8a-dimethyl-1,2,3,7,8,8a-hexahydronaphthalene

Constituent 12

Reference substance name:

rel-(4R,4aR)-4,4a-dimethyl-6-(prop-1-en-2-yl)-1,2,3,4,4a,7-hexahydronaphthalene

Molecular formula:

C15H22

IUPAC Name:

rel-(4R,4aR)-4,4a-dimethyl-6-(prop-1-en-2-yl)-1,2,3,4,4a,7-hexahydronaphthalene

Constituent 13

Reference substance name:

rel-(1R,8aR)-1-isopropyl-4,7-dimethyl-1,2,3,5,6,8a-hexahydronaphthalene

Cas Number:

317819-82-2

Molecular formula:

C15H24

IUPAC Name:

rel-(1R,8aR)-1-isopropyl-4,7-dimethyl-1,2,3,5,6,8a-hexahydronaphthalene

Constituent 14

Reference substance name:

Likely sesquiterpine, MW 202

Molecular formula:

C15H22

IUPAC Name:

Likely sesquiterpine, MW 202

Constituent 15

Reference substance name:

Likely acetylated sesquiterpenes and acetylated sesquiterpene alcohols

IUPAC Name:

Likely acetylated sesquiterpenes and acetylated sesquiterpene alcohols

Constituent 16

Reference substance name:

Likely oxygenated sesquiterpenes

IUPAC Name:

Likely oxygenated sesquiterpenes

Constituent 17

Reference substance name:

Unknown constituent, MW 260

IUPAC Name:

Unknown constituent, MW 260

Test material form:

liquid

Specific details on test material used for the study:

Commercial Name: Vetiveryl Acetate 112 Extra
Givaudan Code No.: 0519103
Aspect: yellow to brownish yellow liquid
Lot No.: VE00389747
Expiration date: September 16, 2017

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (Bois-de-Bay, Satigny, Switzerland) was used.
The sludge is collected in the morning, washed three times in the mineral medium, (by centriguging at 1000g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day.

Duration of test (contact time):

67 d

Initial test substance concentration

Initial conc.:

30 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Results and discussion

Test performance:

Degradation of sodium benzoate exceeded 40% after 7 days and 65% after 14 days: the activity of the inoculum was thus verified (validity criterion).
The repeatability validity criterion (not more than 20% difference between replicates) is fulfilled. Therefore, the test is considered valid.

% Degradation

% Degradation 1

Key result

Parameter:

% degradation (O2 consumption)

Value:

40

Sampling time:

28 d

Remarks on result:

other: mean result

% Degradation 2

Key result

Parameter:

% degradation (O2 consumption)

Value:

53

Sampling time:

67 d

Remarks on result:

other: mean result

% Degradation 3

Parameter:

% degradation (O2 consumption)

Value:

42

Sampling time:

28 d

Remarks on result:

other: Replicate 1

% Degradation 4

Parameter:

% degradation (O2 consumption)

Value:

39

Sampling time:

28 d

Remarks on result:

other: Replicate 2

% Degradation 5

Parameter:

% degradation (O2 consumption)

Value:

62

Sampling time:

67 d

Remarks on result:

other: Replicate 1

% Degradation 6

Parameter:

% degradation (O2 consumption)

Value:

44

Sampling time:

67 d

Remarks on result:

other: Replicate 2

Details on results:

Vetiveryl Acetate 112 Extra undergoes 40% biodegradation after 28 days (53% biodegradation after 67 days) in the test conditions.
Vetiveryl Acetate 112 Extra did inhibit the intrinsic respiration of the inoculum by more than 20% at the test concentration on the first day of the test. Slight toxicity towards the inoculum at test concentration can therefore not be excluded.
Thus, Vetiveryl Acetate 112 Extra should be regarded as not readily biodegradable according to this test.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

inherently biodegradable

Conclusions:

The Ready Biodegradability of Vetiveryl Acetate 112 Extra was determined by the Manometric Respirometry Test. Vetiveryl Acetate 112 Extra undergoes 40% biodegradation after 28 days. Thus, Vetiveryl Acetate 112 Extra should be regarded as not readily biodegradable according to this test. Vetiveryl Acetate 112 Extra did inhibit the intrinsic respiration of the inoculum by more than 20% at the test concentration on the first day of the test. Slight toxicity towards the inoculum at test concentration can therefore not be excluded.

Since degradation had not reached a plateau, the test was extended. A slight difference in behaviour between the two replicates was observed with degradation levels of 44 and 62% being achieved after 67 days. Based on the > 60% result for one replicate, it is considered that vetiveryl acetate and its underlying constituents are inherently and ultimately biodegradable and not expected to be persistent in the environment.