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EC number: 200-609-3 | CAS number: 65-45-2
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Quotation of National Toxicity Program database for experimental results. No experimental data; strains etc. given. Reporting of various QSAR predictions. No validation of QSARs.
Data source
Reference
- Reference Type:
- publication
- Title:
- Prediction of Salmonella mutagenicity
- Author:
- Zeiger E, Ashby J, Bakale G, Enslein K, Klopman G & Rosenkranz HS
- Year:
- 1 996
- Bibliographic source:
- Mutagenesis, 1996, 11(5), 471-484
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- No details in publication
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Salicylamide
- EC Number:
- 200-609-3
- EC Name:
- Salicylamide
- Cas Number:
- 65-45-2
- Molecular formula:
- C7H7NO2
- IUPAC Name:
- salicylamide
- Details on test material:
- - Analytical purity: 99%
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA97, TA98, TA100, TA1535
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced rat and hamster liver S9
- Test concentrations with justification for top dose:
- no data
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- not specified
- Positive control substance:
- not specified
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA97, TA98, TA100, TA1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Several SAR and QSAR methods were applied:
SA (structural alert; Ashby, J., Environ. Mutagen., 1985, 7, 919 -921)
SA+ (structural alert with intervention of expert judgement; Ashby)
CASE (Computer Automated Structure Evaluator, with learning sets from NTP and US-EPA)
TOPKAT
All gave consistent negative (non-mutagenic) predictions for 2 -hydroxybenzamide.
The ke procedure (Bakale G., et al, Carcinogenesis, 1987, 8, 253 -264) gave a questionable result, ke = 3.1
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The study is considered to be suitable for use as supporting information.
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