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Ecotoxicological information

Toxicity to soil microorganisms

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Administrative data

Endpoint:
toxicity to soil microorganisms
Type of information:
experimental study
Adequacy of study:
disregarded due to major methodological deficiencies
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Test conditions and methods poorly described, semi-quantitative and not validated method.

Data source

Reference
Reference Type:
publication
Title:
THE DESTRUCTION OF VANILLIN IN THE SOIL BY THE ACTION OF SOIL BACTERIA.
Author:
ROBBINS WJ; ELIZANDO AE
Year:
1918
Bibliographic source:
ALA. AGR. EXP. SB. BULL. 204. PP.125-31.

Materials and methods

Principles of method if other than guideline:
Amendement of soil with vanillin and soil microorganism quantification.
The effect of vanillin on microorganisms was assessed by measuring the number of microorganisms before and during the incubation period.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
no data

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
not specified

Test organisms

Test organisms (inoculum):
other: aerobic microorganisms

Study design

Total exposure duration:
57 d

Test conditions

Details on test conditions:
The experiment was performed with 2 different soils (Alabama - ALB- and Arlington - ARL- , US). Alabama soil was characterised by the presence of vanillin-destroying bacteria, which were isolated and cultured in mineral media. Arlington soil is a heavy silty clay loam.
An experimental unit was composed by 9 Kg of air dry soil, placed in two-gallon pots and hydrated with distilled water. Each experimental condition was performed in duplicate. The experimental conditions were:

1- ALB
2- ALB + Vanillin [1g/kg]
3- ARL
4- ARL + Vanillin [1g/kg]
5- ARL + Vanillin [1g/kg] + suspension of pure culture of vanillin destroying bacteria

From time to time, the number of microorganisms was determined until 57 days of incubation.

Microorganism concentration determination:
The number of microorganisms was determined by the method described by Brown using albumen agar (no more details were provided by the
authors). The analysis was performed in duplicate using dilution of 1/20000 and 1/200000.
Reference substance (positive control):
not specified

Results and discussion

Details on results:
no data

Applicant's summary and conclusion