Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 680-378-7 | CAS number: 138624-11-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 5-3-03 to 7-3-03
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- sodium;(4-amino-1-hydroxy-1-phosphonobutyl)-hydroxyphosphinate
- Cas Number:
- 121268-17-5
- Molecular formula:
- C4H12NaNO7P2 · 3 H2O
- IUPAC Name:
- sodium;(4-amino-1-hydroxy-1-phosphonobutyl)-hydroxyphosphinate
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot No.of test material: Sponsor and #005Y030
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Dark room at room temperature
- Stability under test conditions: Stable
- Solubility and stability of the test substance in the solvent/vehicle: 40 g/L
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: None
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Final dilution of a dissolved solid, stock liquid or gel: 500 mg/L, 200 mg/L, 80 mg/L, 32 mg/L, 13 mg/L dilutions of the solid in the prepared freshwater were prepared.
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 500 mg/L, 200 mg/L, 80 mg/L, 32 mg/L, 13 mg/L dilutions of the solid in the prepared freshwater
- Sampling method: Physical observation of health of the daphnids
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Controls: A freshwater solution without the substance being tested was prepared as a negative control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): H2O
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): 500 mg/L, 200 mg/L, 80 mg/L, 32 mg/L, 13 mg/L dilutions of the tested solid in the prepared freshwater
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): None
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: Toxikon Corporation, Jupiter, FL
- Age of parental stock: gravid females
- Feeding during test: No
- Amount: 100
ACCLIMATION
- Acclimation period: 24 hours
- Acclimation conditions: Isolatred in food-free dilution water
- Type and amount of food: No food
- Health during acclimation (any mortality observed): No mortalities and were in good health
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
Water fleas will be neonates (i.e., <24 hours old) obtained by isolating gravid females from the culture water in food-free dilution water <24 hours prior to beginning the test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 2 d
- Remarks on exposure duration:
- None
- Post exposure observation period:
- Immediately after the end of the experiment.
Test conditions
- Hardness:
- 70 mg/L as CaCO3 at test intiation
- Test temperature:
- 20.1 to 20.6 degrees celsius during the duratino of the 48 hour exposure.
- pH:
- 5.7 to 7.8 during the duration of the 48 hour exposure
- Dissolved oxygen:
- Initial dissolved oxygen concentrations in the control and test solutions were 8.8 to 8.9 mg/L and 8.4 to 8.7 mg/L at test termination.
- Salinity:
- 26 mg/L as CaCO3
- Conductivity:
- 399 microS at test intiation
- Nominal and measured concentrations:
- Nominal Concentrations: 500 mg/L, 200 mg/L, 80 mg/L, 32 mg/L, 13 mg/L dilutions of the solid in the prepared freshwater
- Details on test conditions:
- TEST SYSTEM
- Test vessel: vials
- Type: closed
- Material, size, headspace, fill volume: Glass, 50 mL, none, 50 mL
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Water origninated in Town of Jupiter
- Pesticides: <1.040 microg/L
- Chlorine: 87 mg/L
- Alkalinity: 70 mg/L as CaCO3
- Ca ratio(mg/L): 19 mg/L
- Conductivity: 399 microSiemens
- Salinity: 26 mg/L as CaCO3
- Intervals of water quality measurement: Beginning and end of test
OTHER TEST CONDITIONS
- Photoperiod: 16 hours of light / 8 hours of dark
- Light intensity: 13.2 to 13.9 microMls per square meter per second
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Dissolved oxygen content, alkalinity, hardness, and conductivity. These were measured at the beinning and end of the test. pH and temperature were measured continuously for the duration of the test.
VEHICLE CONTROL PERFORMED: Yes
RANGE-FINDING STUDY
- Test concentrations: 100 mg/L and 1000 mg/L
- Results used to determine the conditions for the definitive study: Yes - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 1 d
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 350 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 2 d
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 170 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 2 d
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 80 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Behavioural abnormalities: None
- Mortality of control: None - Reported statistics and error estimates:
- 95% confidence was used to determine the 1 and 2 day EC50 values. The 95% confidence range for the 1 day EC50 value is 290 to 420 mg/L. The 95% confidence range for the 2 day EC50 value is 130 to 210 mg/L. This and the 2 day NOEC value were calalculated with the computer program ToxCalc.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- This test effectively estabilshes EC50 24 hour and 48 hour and NOEC guidelines for short term toxicity to aquatic invertebrates based on the OECD 202 guideline.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
